麻痹性贝类毒素细胞检测法的建立与评价-流行病与卫生统计学专业毕业论文.docxVIP

麻痹性贝类毒素细胞检测法的建立与评价ABSTRACT 麻痹性贝类毒素细胞检测法的建立与评价 ABSTRACT Objective：Paralytic shellfish poisoning(PSP)is known as neural paralysis by in-take of shellfish with poisons by mistake．PSP is widely distributed in the oceans all over the world．PSP iS characterized with strong toxicity and high mortality．PSP functioned as inhibitor of sodium channel inside human body．It targets to cardiovascular and neuron system with high specifity．There is no effective antidote of PSP SO far． Many testing methods for PSP were established，however costs of these methods are still very hi曲．Thus there is an urgent need for a PSP testing method with high sensitivity and low cost． Methods：This study aims to establish an N2a cell based method to test PSP in shellfish with high sensitivity and low cost．Data of poisoning incidents by in-taking of PSP within 1 5 years were collected and analyzed． The epidemiological distribution of PSP poisoning was studied and discussed．2D-DIGE was applied to screen the differentiated proteins induced by PSP in N2a cells the key proteins were then further validated by Western—blot analysis and ELISA．The testing method is based on the validated proteins and specifity and sensitivity of this method Was evaluated． Results：There Was 2 1 references used in this study,a total of 1 3 1 PSP poisoning cases were reported with 889 people involved and 69 were III 万方数据 硕士学位论文dead，the 硕士学位论文 dead，the mortality rate was 7．76％．1 30 cases were in the coastal area．3 cases were reported in the first quarter,67 in the second quarter,59 in the third quarter and 2 in the fourth quarter．1 24 cases were caused by Nassariidae，including 732 patients．Proteomic study revealed that STX induced 9 proteins abnormally altered in N2a cells．The expression of 1 4-3—3，a—enolase and cofilin一2 were validated by ELISA．The expression of泓enolase was positive correlated with the concentration of STX，and the results were consisted with classic methods for testing PSE Conclusion：PSP poisoning cases were mai