应用微透析技术和高效液相色谱法测定川芎嗪对大鼠脑内.pdfVIP

906 药学学报 Acta Pharmaceutica Sinica 20 13, 48 (6): 906 910 用微透析技术和高效液相色谱法测定川芎嗪对 大鼠脑内多巴胺释放量的影响 * 吕允凤 ( 国家食品药品监督管理局医疗器械技术审评中心, 北京 100044) 摘要: 利用脑内微透析技术与现代分析方法, 考察了不同剂量川芎嗪对大鼠脑内多巴胺释放的影响。采用脑 内微透析技术进行在体动态取样, 建立了高效液相 电化学检测方法测定脑透析液中的多巴胺含量。结果显示川 芎嗪皮下给药能够剂量相关性地增加大鼠脑内不同脑区中多巴胺的释放。 方法能够在不影响动物正常生理活 动的情况下准确反映药物对大鼠脑内多巴胺释放量的影响, 与传统神经递质研究方法相比, 具有明显的优势。 关键词: 川芎嗪; 脑微透析; 高效液相-电化学检测; 多巴胺 中图分类号: R9 17 文献标识码: A 文章编号: 0513-4870 (20 13) 06-0906-05 Effects of ligustrazine on extracellular dopamine levels in rat brain dialysate LÜ Yun-feng* (Centerfo r Medical Device Evaluation, State Food and Drug Administration, Beijing 100044, China ) Abstract : Using brain microdialysis and LC-ECD, the content of dopamine in rat brain was detected to investigate the effects of ligustrazine. A liquid chromatography-electrochemical detector method has been established and validated for the determination of dopamine in rat brain dialysate. The results indicate that ligustrazine administration by subcutaneous inject ion significantly increased dopamine release in rat medial prefrontal cortex, nucleus accumbens and hippocampus in a dose-related manner. The drug ’s effects on dopa release in rat brain could be directly detected by microdialysis combined with HPLC-ECD and this method has the preponderance over traditional neurology methods. Key words: ligustrazine; brain microdialysis; HPLC-ECD; dopamine 微透析 (microdialysis) 技术是一种在体化微量 量生物活性物质将 浓度梯度从膜外逆向扩散入膜 采样技术, 采用具有一定截留分子量的纤维半透膜