氯化血红素与环糊精及白蛋白相互作用的研究-食品科学专业毕业论文.docxVIP

摘要摘 摘要 摘 要 为了改善氯化血红素水溶性差，遇光、氧易分解这一缺点，本研究利用p．环糊精对 其进行包合，形成氯化血红素．13．环糊精包合物。p．环糊精具有外亲水，内疏水的特殊结 构，能与结构和性质适合的化合物形成包合物以增加其稳定性和水溶性。本文采用微波 法制备包合物，并以包合率为指标测定了最优化的工艺条件。通过紫外，红外光谱鉴定 了包合物的形成。 本论文采用荧光光谱和紫外吸收光谱法研究了氯化血红素与牛血清白蛋白的相互 作用。实验结果显示氯化血红素对牛血清白蛋白的内源荧光有较强的静态荧光淬灭作 用；得出了反应的结合常数K=2．48x10 L／mol、结合位点n=1．25；根据F／Srster的偶极．偶 极非辐射能量转移理论，计算出结合位置距离牛血清白蛋白212位色氨酸残基0．99 nml 使用三维荧光光谱及同步荧光光谱法探讨了氯化血红素对牛血清白蛋白构象的影响。 本论文采用荧光光谱研究了胆红素与牛血清白蛋白的相互作用。实验结果显示胆红 素对牛血清白蛋白的内源荧光有较强的静态荧光淬灭作用；得出了反应的结合常数 K=4．9x 104 L／mol、结合位点n=0．74；根据F6rster的偶极．偶极非辐射能量转移理论，计 算出结合位置距离牛血清白蛋白212位色氨酸残基2．12 nml使用同步荧光光谱法探讨 了胆红素对牛血清白蛋白构象的影响。 关键词：氯化血红素，13一环糊精，包合物，白蛋白，荧光，胆红素 摘要Abstract 摘要 Abstract In order to improve the defects such as easiness to decompose meeting light or oxygen and poor water-solubility，heimin Was included by 13-cyclodextrin to form heimin-13一 cyclodextrin inclusion．13-cyclodextrin has characteristic structure wi廿l hydrophilic outer surface and hydrophobic inner surface and Can increase the stability and water-solubility of other compounds by forming inclusion with them．In this study，microwave method was employed to produce inclusion composed of lB-cyclodextrin and other compounds．Take inclusion ratio as object，the process of conclusion Was optimized．The inclusion was identified through UV and IR spectrometry． The interaction between bovine serum albumin and heimin was investigated by fluorescence spectra and UV．Experimental results indicated that the fluorescence quenching of heimin to bovine serum albumin Was static quenching with the binding sites is 1．25 and the equilibrium constant K is 2．48 1 0’L／m01．The binding locality was 0．99 rl／n away from tryptophan residue-2 12 in bovine serum albumin based on F6rster’S non—radiation energy transfer mechanism．The effect of heimin on the conformation of bovine serum albumin Was analyzed by three—dimensional fluorescence spectra,contour spectra and synchronous spectra． The binding of bilirubin to bovine serum albumin Was studied using fluorescence spectra． Experimental