抗金黄色葡萄球菌海洋微生物的分离、发酵及产物的初步纯化-作物遗传育种专业毕业论文.docxVIP

!旦垫望坚些型兰堕：兰堕垫堂堡些奎兰 !旦垫望坚些型兰堕：兰堕垫堂堡些奎兰 !塑!堡堡主堕窒生 采用有机剂抽提法、酸碱抽提法、大孔吸附树脂柱层析和阳离子交换树脂柱 层析等方法对HSL I一306菌株发酵产物进行了初步分离纯化。 关键词：海洋微生物，分离，鉴定，发酵，抗生素，层析 廖文彬 廖文彬 抗金黄色葡萄球菌海洋徽生物的分离、发酵及产物初步纯化 ABSTRACT Since Flemming discovered penicillin，more than 350 kinds of antibiotics have been used in clinic，which makes many epidemic diseases controlled．However,people must find new drug continuously becaBse of the wide use of antibiotics and the emergence ofbacterial drug resistance． Biological reSOUrceS in land once were the main s01．1rl弛of antibiotics．Howeveg with the research going on，it is becoming more and more difficult tO find new antibiotics from biological resources in land．Sea and oc啪has particular environments，SO marine biology has more blological diversity than terrestrial biology． Marine biology is becoming one ofthe hotspots ofthe new drug research in the wodd． 1,451 marine microorganie strains were isolated from 127 selected marine environment specimens in 1 9 location／of Hainaa Island and surrounding areas，including 656 bacterial strains，419 aetinomycetie smlins and 376 fungal strains．1，451 strains fermentation products were t=tea by agar difussion method to screen the antibacterial bioactive substances．As a result,13 mieroorgmie metabolitcs have prominent antibacterial bioactivity towards Stapha／ococcus口“nr埘。 HSL I一306 WaS a aetinomycete isolated from the mire of mangrove in Hainan,ngar diffusion method showed the metalmlities of the staain had prominent bioactivity inhibiting Staphalococcus allreus，and the antibacterial bioaetivity call not be lost after screening again．The taxonomic investigation 011 nutritional and morphological characters display the HSL I一306 stnfm is spirillospora sp．． The fermentation condition and gradients of the medium have been studied，the results showed the optimum fermentation condition w讹船hr ofinoculation age．96hr ofthe fermentation time and an initial pH of6．5～7．2．on that condition．Orthogonal test experiment showed the best culture condition an