一株生物表面活性剂产生菌的分离及其特性研究-微生物学报-中国.PDFVIP

微生物学通报 AUG 20, 2009, 36(8): 1110~1116 Microbiology © 2009 by Institute of Microbiology, CAS tongbao@ 研究报告 一株生物表面活性剂产生菌的分离及其特性研究 孙 燕 洪 青* 李顺鹏 ( 210095) 摘 要: 模炼油厂污泥中分离得到 1 株生物表面活性剂产生菌 C-3, 根据其生理生化特性和 16S rDNA 序列相似性分析, 将其鉴定为铜绿假单胞菌(Pseudomonas aeruginosa) 。初步研究了其产生物 表面活性剂的最适条件, 在以植物油为碳源、30°C 、初始 pH 8 、Ca2+ 浓度 20 mg/L 、250 mL 三角 瓶中装 75 mL 发酵液的条件下, 最利于菌株的生长和生物表面活性剂产生。它的成分为糖脂类物 质, 临界胶束浓度(CMC)为 50 mg/L, 具有很好的增溶效果。 关键词: 生物表面活性剂, 分离, 最适条件, 成分分析 Isolation and Characterization of a Biosurfactant- producing Bacterium * SUN Yan HONG Qing LI Shun-Peng (Key Laboratory of Microbiological Engineering Agricultural Environment, Ministry of Agriculture, College of Life Science, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China) Abstract: A biosurfactant producing bacterial strain C-3 was isolated from the sludge of a petroleum refin- ery and identified as Pseudomonas aeruginosa. The optimal conditions for the growth and biosurfacant pro- duction of strain C-3 were investigated. The results showed that strain C-3 could grow well and produce the largest amount of biosurfacant under the following conditions: seed-oil as sole carbon source, 30°C, initial pH 8, Ca2+ concentration of 20 mg/L, 75 mL medium in 250 mL Erlenmeyer flask. The biosurfactants pro- duced by strain C-3 was identified as lipopeptides, showing high surface activity and emulsifying efficiency with critical micelle concentration (CMC) being 50 mg/L. Keywords: Biosurfactant, Isolation, Optimal conditions, Biosurfactant analysis (Biosurfactant) [2