抗豆象基因等位性鉴定及栽培绿豆V2709抗豆象遗传与分子标记-作物遗传育种专业毕业论文.docxVIP

AbstractMungbean(Hgna Abstract Mungbean(Hgna rad／ata(L．)Wilczek)belongs to＆删D嘲呻and it is popularly planted in south Asia．Bruchid is main pest of mungbean and can bring great harm to mungbean production and storage．So，resistance variety is an important way to prevent brucIIid．In present study,the major objectives were to stody the inheritance of resistance to the cultivar mungbean，V2709 and to tag the resistance gane by molecular markers．in order to芦odde useful information for resistance breeding and fine mapping of resistant gone．11圮main results we∞鼬follows： 1．By using the bmchid resistant明ltivar V2709，the susceptible∞珩VⅡVCl973A’and their F2，F3 to study the inheritance ofbmchid resistance in V2709．The reSUIts were as follows：Fi individuals well： 棚resistant to bmcllis，the segregation in F2and BClFl population were 3R：IS and 1R：IS respectively． F3 population segregated in 1：2：1 ratio．It indicated that the brucMd resistance of V2709 is mainly controlled by dominant major gone． 2．Five mungbean accessions with resistance to bmcbid，V2802．V2709。VC6089A-6，ACCAl， and VCl973A，V2709．2．were crossed with each other to test the allelism of the resistance genes． Segregation in the F2 populations of bmchid resistance from(vCl973A／V2709-2)xACCAI， VC6089A-6×V2709 and VC6089A-6×V2802 were suggested that the resistance genes between VCl973A，V2709-2 and Acc41：between VC6089A-6 and V2709；between VC6089A-6 and V2802 werenon·allelicwhilethesegregationinF2populationsofthe crOSseS(VCl973A，V2709-2)xV2802． (VCl973ⅣV”09-2)xV2709 indicated that the resistant gone in them allelic closely linked． 3．At the same time，63 RAI D markers，100 SSR and 28 STS primers were used for BSA arial3，sis to find molecular marker that link with the resistant gene．As the reSUIts．OPC-06 and STSbr2 we犯 identified to be related with the brucMd resistant gene．After verified in F2 individuals，the genetic distances between these two markers and the target gone were 11．0cM and 5．8cM respectively． Key word：mungbean，bru