米糠中阿魏酸的提取分离与测定研究-分析化学专业毕业论文.docxVIP

中南大学硕士学位论文上样流速为1．0BV／h，浓度为1 中南大学硕士学位论文 上样流速为1．0BV／h，浓度为1 800～2300mg／L，洗脱流速为2．0BV／h (乙醇：水：盐酸=65：3l：4，v／v)。洗脱液浓缩、干燥后得到白色 样品，且样品与阿魏酸对照品红外光谱图一致。 4．采用Fenton反应体系，研究了阿魏酸对羟基自由基的清除作 用。随着阿魏酸浓度增大，清除作用增强。其在低浓度时即有很好的 清除率，且在浓度较低的时候清除效果要好于另两种优良的羟自由基 清除剂抗坏血酸和硫脲。 关键词： 米糠，阿魏酸，提取工艺，高效液相色谱法，荧光光谱法 Ⅱ 中南大学硕士学位论文 中南大学硕士学位论文 ABSTRACT ABSTRACT Rice bran is the byproduct of rice，and our country produces more than 10 million tons rice bran per year．Because it has many kinds of active substances，such as femlic acid,which iS valuable，race bran has become one of the focus food and nutrition research during the past several years．However,there are many problems existing in practice． such as no rapid product analytical and evaluation method,instability of product，long production cycle，low utilization，etc．ne following aspects have been done in our research to solve the problems listed above： 1．According to the properties of Ferulic acid in structure and character,RP．HPLC analytical method and Spectrofluorimetry were developed for determination of Ferulic acid．Effects of mobile phase constitute，pH and temperature on retention behaviors of Ferulic acid in column were discussed．111e optimal chromatographic conditions included oDS-Cis column(4．6 mmx l 50 mm)and the mobile phase consisting ofa mixture ofmethan01．water-acetic acid(35：65：0。9，volume rati∞at a flow rate of 1．0 mL／min at 25℃．111e Uv detection wavelength Was at 322nm．There iS a good linear relationship in the range of 1．O～ 5．OIlg／mL for ferulic acid．and the correlative coefficient is 0．99928．The relative standard deviation is 0．98％and the average recovery is 99．7％． For Spectrofluorimetry,the excitation and emission wavelength iS 344nm and 362nm．respectively．Influences of different solution and time on tlle fuorescence intensity Wele studied．The average recoveries ofthe method were 99．0％and the coe伍cients of variation were 1．32％．The linear ran