棉花黄萎病抗性基因的关联作图及cDNA文库的构建-细胞生物学专业毕业论文.docxVIP

AbstractLinkage Abstract Linkage disequilibrium mapping of a Verticillium dahliae resistance gene and construction of a SSH Library in cotton Abstract Cotton Verticillium wilt，a disease of cotton caused by Verticillium dahliae，is a kind ofworldwide spread disease that is difficult to be controlled in cotton production． Development of cotton resistant cultivars carl provide all effective and economical method for controlling Verticillium wilt．Therefore，researches on deeply understanding of expression rules about resistance gene and the molecular mechanism about interactions between Verticillium dahliae and the tlost would provide theoretical foundation for developing resistant cultivars． The objectives of the research were attempted to screen molecular marker and candidate genes associated with resistance gene．(1)tlli时一four cotton cultivars coming from different sources were selected to detect the SSR molecular marker linked with Verticillium wilt resistance gene，including twenty—two resist cultivars and twelve susceptible cultivars．The resistance of the cultivars was determined in the field disease nursery,and the result verifies the original resistance． Five hundred pairs of SSR primers covering the whole cotton genome were selected to genotype all cultivars．Out of these primers，three hundred and eighteen pairs of SSR primers showed polymorphic between the cultivars．By cluster analysis with regional fuzzy clustering algorittma，the tested varieties could be divided into two groups，and the result is related to resistance of every cultivar．Then—linkage disequilibrium mapping method was used to test an association between SSR markers and Verticiltium resistance gene，with SAS software，the result showed that fourteen markers are significantly related to resistance gene at P0．001 level． U Abstract(2)To Abstract (2)To obtain resistant-related candidate genes of Verticillium wilt fiom upland cotton,a mgh Verticillium wilt resistant cotton eultivar(changkangmian)was used to