落射荧光显微术高通量分析单细胞中的酯酶-分析化学专业毕业论文.docxVIP

I』东人中硕I‘中f●论定Abstract I』东人中硕I‘中f●论定 Abstract This thesis consists of three parts：I．Determination of esterase activity by epi—fluorescence microscopy． II．Determination of esterase activity by hi曲一throughput single-cell epi-fluorescence spectroscopy．III．A high-throughput fluorescence spectroscopy method with a microfluidic chip for single-ceU fluorescence． In Chapter I，the esterase activity in the extracts ofMGC803 was determined by cpi·fluorescence microscopy．In this part FDA Was used as the enzyme substrate．A enzyme catalytic reaction occurred．The resultant was excited by a 488 mn argon-ion la