毛白杨韧皮部次生代谢物质提取分离及对光肩星天牛抗性的研究-森林保护专业毕业论文.docxVIP

些物质在加杨中没有被发现。 关键词=光肩星天牛:毛白杨:加拿大杨:次生物质:提取分离:抗虫性 The The research on secondary material in phloem Populus torncBtosa’S isolation and the resistance to Anoplohora glabripcnni7 S larvae Postgraduate：Bi Yongguo Specialty：Forest Protection Tutor：Prof．Wang Zhigang Prof．Huang Dazhuang Abstract Secondary material in poplar has important and practical significance probing the mechanism of poplar resistance longborn beetle and nurturing the new insect—resistant species the research of resistance Anoplohora glabripenni．Phlom of Populus tomentonsa is extracted all and systemically by the systemical extractation of pesticidal component in plant research the insect—resistant effect of secondary material in poplar tree．In according the different character，we take the different proc and phloem of P．tomcntonsa is extracted three parts：component I、component II and component III．Then we dispose the branch of Populus canadensis，inoculate Anoplohora glabripenni larvae the branch and make P．canadensis be comparism．The insect—resistant effect of the three component is proved through the mortality development and higher weight of Anoplohora glabripenni larvae in branch．The effective component III is isolated by High Performance Liquid Chromatography(HPLC) and the answering component in P．canadensis is isolated．We find the special material in P．tomentonsa through comparing chromatography．We get the following results． 1 Every component in phloem of P．tomcntonsa has toxic effect Anoplohora glabripenni larvae．But the effect is different．The morotality of component I、 II、III is 29．5％、42％and 80．3％respectively．The morotality of comparison is 16．2％．So the toxic effect of component III is the best Anoplohora glabripenni larvae． 2 Every component has different toxic effect the different instar Anoplohora glabripenni larvae．The effect is the best first instar larvae，the effect declines apparently second instar larvae and third instar larvae isn’t dead． 3 Every component has apparent restrain