芒果叶绿素提取工艺、性质及芒果苷含量测定的研究-化学工艺专业毕业论文.docxVIP

II II 芒 果 叶 提 取 物 有 与 叶 绿 素 a 和 芒 果 苷 有 相 似 的 紫 外 - 可 见 光 谱 的 特 征 吸 收 峰 。 TG-DTG-DSC分析结果显示：热解反应进行得较为平稳，热分解过程中伴有燃烧过程， 可能有CO2等气体释放。 关键词：芒果叶绿素 芒果苷 提取工艺 稳定性研究 含量测定 III III STUDY ON CHLOROPHYLL EXTRACTION PROCESS FROM MANGO LEAVES, CHLOROPHYLL PROPERTIES AND MEASUREMENT OF MANGIFERIN CONTENTS Abstract In the thesis, chlorophyll was extracted by the ultrasonic-assisted method from mango leaves. The conditions of extraction techniques of chlorophyll were optimized. And the stability of the extracted chlorophyll was studied. A method was developed for determination of chlorophyll and mangiferin in mango leaves by UV-Vis, which could be used to evaluate the quality of mango leaves as medicinal materials. The contents of chlorophyll and mangiferin in mango leaves of the 11 mango cultivars in Baise City were determined. The chlorophyll was preliminarily purified with D101 macroporous resin, which was analysed by UV-Vis, FT-IR, and TG-DTG-DSC. Studying on the extraction process of chlorophyll in mango leaves. Chlorophyll was extracted by ultrasonic-assisted organic solvent method with mango leaves as raw material. Based on single-factor research, the impact of ultrasonic temperature, ultrasonic power, solid-liquid ratio, and ultrasonic time on the extraction effects were explored by orthogonal test. The optimized extraction conditions were 95% ethanol as extractant, solid-liquid ratio of 1:8 (g/mL), ultrasonic temperature, power and time as 60℃, 160W and 30 min respectively. The extraction efficiency ration was 23.6% higher than the conventional dipping extraction method. Studying on the stability of chlorophyll in mango leaves. Chlorophyll in mango leaves was extracted with 95% ethanol. Then its tolerance towards light, temperature, pH value and oxidant, as well as the impact of different metal ions on the stability were studied. The results were as follows: the stability of chlorophyll in mango leaves apparently was affected by Light, pH, value and high temperature.