珍稀濒危物种堇叶紫金牛高效快繁体系的建立.docVIP

珍稀濒危物种堇叶紫金牛高效快繁体系 的建立 孙英坤胡绍庆 庞基良高凯 刘华红陈焕伟 姚涛 陈林敬沈柏春 杭州市园林绿化股份有限公司浙江理工大学建筑工 程学院杭州师范大学生命与环境科学学院龙泉市 林业科学研究院 摘耍： 筛选堇叶紫金牛(Ardisia violacea)野生优株，以其当年新发带休眠腋芽茎 段为外植体，通过启动培养、丛生芽诱导增殖、壮苗培养、生根培养和炼苗移栽 等过程建立其组培快繁技术体系。研究结果表明，最佳启动培养基为MS+O. 80 mg ? L_1KT+O. 10 mg ? I/1 NAA+0. 10 mg ? I/1 TBA,腋芽萌发率达 92. 60%;最佳丛 生芽诱导增殖培养基为MS 1+0. 50 mg ? L1 TDZ+O. lOmg ? L_1 NAA,平均增殖系数 达8?60;最佳壮苗培养基为MS+1.00 mg - L~l KT+O. 50 mg ? L_l NAA;最佳生根培 养基为 1/2MS+2. 00 mg ? L_1 1BA+1. 00 mg ? L_, NAA+1. 00 mg ? L1 AC,平均生根 率达98.70%;采用松鳞和泥炭(2:1, v/v)作为炼苗基质，炼苗成活率可达 85. 30%。实验成功建立了堇叶紫金牛高效组培快繁技术体系，经验证该体系能够 满足规模化生产的需求。 关键词： 堇叶紫金牛;启动培养;丛牛芽; 作者简介:沈柏春 E-mail :498548490@qq. com 收稿日期：2016-11-30 基金：浙江省花卉新品种选育重大科技专项(NO.2012C12909-9) Establishment of a Tissue Culture and Propagation System for Ardisia violacea, a Rare and Endangered Species Yingkun Sun Shaoqing Hu Jiliang Pang Kai Gao Huahong Liu Huanwei Chen Tao Yao Linjing Chen Bochun Shen Hangzhou Landscaping Incorporated; College of Civil Engineering and Architecture, Zhejiang Sci-Tech University; College of Life and Environmentai Sciences, Hangzhou Normal University; Longquan Forestry Research Institute; Abstract： Shoot segments with axi1lary buds from young branches of wiId Ardisia violacea with excellent characters were cultured in vitro. Through bud induetion, multiple shoots proliferation, subculture, rooting and transplantation, a regeneration system was established. The optimal bud induction medium was MS+0. 80 mg ? L 1 KT+0. 10 mg ? L 1 NAA+0. 10 mg ? L 1 IBA, and the germ in ati on freque ncy of axi 1 lary buds reached 92. 60%; the optimal multiple shoot proliferation medium was MS 1+0. 50 mg ? L1 TDZ+0. 10 mg ? L1 NAA, and the average proliferation coefficient reached 8. 60; the optimal subculture medium was MS+1. 00 mg ? L 1 KT+0. 50 mg ? L 1 NAA; the optimal rooting medium was 1/2 MS+2. 00 mg ? L 1 IBA+1. 00 mg ? L 1 NAA+1. 00 mg ? L 1 AC, and the average frequency of shoot rooting reached 98. 70%. Using pine bark: peat (2:1, v/v) as a matrix, the transplant