慢性束缚应激抑郁症大鼠模型的复制及逍遥散对海马色氨-环球中医药.PDFVIP

摇1198 环球中医药2017年11月第10卷第11期摇 Global Traditional Chinese Medicine,November2017,Vol郾10,No郾11 ·论著 · 慢性束缚应激抑郁症大鼠模型的复制及 逍遥散对海马色氨酸代谢途径的调节作用 焦海燕摇 严志祎摇 姜幼明摇 刘玥芸摇 陈家旭 揖摘要铱摇 目的摇 通过慢性束缚应激方法复制大鼠抑郁症模型,并观察海马色氨酸代谢途径的 变化与逍遥散的调节作用。 方法摇 将24 只雄性SD大鼠随机分成正常组、模型组、逍遥散组与氟西 汀组。 采用21天慢性束缚应激的方法复制大鼠抑郁症模型,通过大鼠体重变化、糖水偏好实验、新 环境抑制进食实验、旷场试验对模型进行评价。 采用 ELISA 法测定各组大鼠海马色氨酸 (tryptophan,Trp)与5鄄羟色胺(5鄄HT)的含量。 结果摇 模型组大鼠糖水偏好量和新环境进食时间与正 常组大鼠相比有显著差异(P0.01);旷场试验的中央区进入次数、中央区停留时间以及总穿格次数 与正常组相比均有统计学差异(P0.01,P0.05)。 ELISA结果显示,模型组大鼠海马5鄄HT含量显 著低于正常组(P0.01),而逍遥散组和氟西汀组海马5鄄HT 含量明显高于模型组(P0. 05, P0.01);模型组大鼠海马中色氨酸的含量较正常组明显增加(P0.01)。 结论摇 在慢性束缚应激 抑郁症大鼠海马中存在色氨酸代谢途径的异常,这与抑郁症的发病密切相关,且逍遥散能够通过对 海马色氨酸代谢的调节起到一定的抗抑郁作用。 揖关键词铱摇 抑郁症;摇 色氨酸代谢途径;摇 5鄄羟色胺;摇 逍遥散 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2017郾11郾003 Model of depressionratswithchronicimmobilizationstressandeffectsofXiaoyaosan onthechange of tryptophan metabolism pathway摇 JIAO Haiyan,YAN Zhiyi,JIANG Youming,et al. 摇 Institute of TCM,Beijing University of Chinese Medicine,Beijing 100029,China Corresponding author:CHEN Jiaxu,E鄄mail:chenjx@ 揖Abstract铱摇 Objective摇 To establish the depression rat model by chronic immobilization stress method,andobservethechangeof tryptophanmetabolismpathway inhippocampusandtheregulationofXi鄄 aoyaosan. Methods摇 24 SD ratswere randomly divided into normal group,model group,Xiaoyaosan group andfluoxetine group,the21days depression rat model was made by chronic immobilization stress method, and the model was evaluated by bodyweight change,sucrose preferencetest,novelty suppressedfeeding test and open field test. ELISA method was used to determine the contents of tryptophan and 5鄄HT in hippocampus. Results摇 The results of sucrose preference test and novelty suppressed feeding test showed that there was a significant difference between model group and normal group (P0.01),the central