抗氧化基因和抗氧化酶表达对精液质量的影响-泌尿外科专业毕业论文.docxVIP

广州医科大学抗氧化基因和抗氧化酶表达对精液质量的影响 广州医科大学 抗氧化基因和抗氧化酶表达对精液质量的影响 PAGE PAGE 10 Effect of expression of Antioxidant genes and antioxidant enzymes on semen quality M. Dcandidate: Kang Chen Supervisor: Xingcheng Gao Bolan Yu Major: urology Abstract Human infertility is a common medical problem, affecting about 10% -15% of all couples worldwide. semen quality is an important etiological factor for infertility. Recent reports have suggested that sperm concentration has declined by almost 50 percent during the past 60 years. Reactive oxygen species (ROS) are one of the most important factors contributing to male infertility. Although small amounts of ROS are involved from the spermatogenesis to zona pellucida penetration, excessive levels can negatively impact the quality of spermatozoa and impair their overall ability to fertilize ova. Studies performed on infertile men have demonstrated that smokers present a significant increase in the concentration of reactive oxygen species (ROS) in semen compared with nonsmokers. Antioxidant defense system plays a very important role in spermatogenesis. Investigating the expression of antioxidant gene and Enzyme of semen may provide new insight into molecular mechanisms of male infertility. Objective Investigating effect of smoking on semen quality. Study of the Correlativity between antioxidant gene expression and semen quality. Method Epidemiological survey of smoking status, clinical data and semen parameters of 1368 people. Detection of seminal plasma SOD enzyme activity and total protein quantitative of 194 normal controls and 228 cases with low sperm vitality. Detection the expression level of sperm antioxidant enzymes and antioxidant gene of 65 normal controls and 55 patients with low sperm vitality. Result the sperm vitality, sperm density, sperm survival rate and semen volume were significantly decreased in heavy smoking group, but the SDI, TZI index and sperm deformity rate were significantly higher compare to nonsmoking