黄连吴茱萸配伍对溃疡性结肠炎大鼠模型的实验研究-方剂学专业毕业论文.docxVIP

中文摘要 目的：从结肠粘膜屏障修复角度探讨黄连吴茱萸配伍对溃疡性结肠炎的作 用，探讨作用机理，拓展其配伍应用，筛选实验效果较好的配伍比例，为临床治 疗溃疡性结肠炎提供实验依据。 方法：通过 TNBS 乙醇混合造模法对 SD 大鼠进行灌肠注射，建立溃疡性结 肠炎模型。观察各组大鼠行为学状态、粪便情况、以及体重的变化，连续给药 10 天后颈椎脱臼处死大鼠，取结肠组织 8cm，测定结肠指数，制作结肠组织切 片（HE 染色），观察组织变化情况；结肠组织匀浆，测定结肠中 TNF-α、EGF、 bFGF 含量，探索黄连吴茱萸配伍对溃疡性结肠炎大鼠肠粘膜屏障的修护作用。 结果：黄连组和黄连吴茱萸不同配伍比例组动物体重较模型组升高，精神状 态恢复明显，结肠部病变较模型组有不同程度的减轻。黄连组、吴茱萸组和连萸 2：1 组与模型对照组比较，TNF-α 表达降低；吴茱萸水煎液组和连萸 1：1 组 EGF 表达较模型显著升高；吴茱萸水煎液组、连萸 1：2 组以及连萸 2：1 组 bFGF 表 达较模型升高，其中连萸 2：1 组与模型组比较差异最为显著。 结论：黄连吴茱萸配伍组合可以改善溃疡性结肠炎模型大鼠脓血便症状，对 其肠粘膜屏障有一定修复作用，通过对 EGF、bFGF、TNF-α表达的观察，得到 黄连吴茱萸 2:1 为最佳配伍比例，主要通过降低 TNF-α，升高 EGF、bFGF 的协 同表达促进肠粘膜屏障的修复作用。结合实验动物的粪便情况及相关实验结果， 初步判定本实验所建立的溃疡性结肠炎动物模型可能属于中医湿热证的范畴。 关键词:黄连吴茱萸配伍；溃疡性结肠炎；TNF-α；EGF；bFGF I 成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 II II ABSTRACT OBJECTIVE: The research on the roles of different compatibility of Huanglian and Wuzhuyu in repair of colonic mucosa with ulcerative colitis is to the investigate the mechanism, expand the apply of the compatibility of Huanglian and Wuzhuyu, find out the best compatibility of Huanglian and Wuzhuyu based on the effect of experiments, which provides the experimental evidence to clinical treatment on ulcerative colitis. METHODS: Trinitro-benzene-sulfonic acid(TNBS) dissolved in ethanol was administrated intrarectally in Sprague Dawley rats to induce experimental ulcerative colitis. The model rats were randomly divided into eight group: groups ：three Huanglian and Wuzhuyu groups treated with different compatibility of Huanglian and Wuzhuyu group, Huanglian group, Wuzhuyu group, model group, blank group, and GM treatment group. Observed in rats for 10 day on vital signs, excremental condition, and body weight, a detailed record, respectively. 10 days later, all animals were sacrificed, sacrificed, 8cm intestine tissure were obtained, Intestines weight index，body weight，TNF-α, EGF, bFGF of all the groups were assayed. RESULTS: After the ulcerative colitis model was established, compared with the treatment in model group, the bo