麻醉对罗非鱼和金鲳鱼离水保活的影响-农产品加工及贮藏工程专业毕业论文.docxVIP

II II 明该浓度的麻醉剂是最优的麻醉保活浓度，其能使鱼在保活过程中存 活最久。因此，二氧化碳和 MS-222 是较为理想的水产麻醉剂，能够 应用于水产运输中。 关键词：鱼 麻醉 离水保活 应激反应 III III EFFECT OF ANESTHETIC ON TRANSPORTING LIVE TILAPIA AND GOLDEN POMPANO WITHOUT WATER ABSTRACT In this paper, two kinds of fishes, which are tilapia and gold pomfret, are anesthetized by using two kinds of anesthetics, which are carbon dioxide and MS-222(Tricaine methane sulfonate), for keeping alive without water. This paper studies the anesthetics affect on fishes, and researches the impact on fishe properties, which include blood electrolyte concentration, biochemical indicators and cortisol. The results are as follows: In the process of anesthetizing by using carbon dioxide in high concentration(pH= 5.6) at 15 ℃ , it spends the minimum time for tilapia into anesthesia, and only takes 171 seconds into the third stage of anesthesia. While using MS-222 anesthetizes tilapia, the anesthesia time decreases and the recovery time increases with the growth of anesthetics concentration. However, when the anesthetics concentration of MS-222 is greater than 100mg/L, the fish irritability and the recovery time will increase. It reveals that the pH of the effective anesthetic concentration of carbon dioxide is 5.6 and the effective anesthetics concentration of MS-222 is 100mg/L. Tilapia and golden pompano by high concentrations of carbon dioxide and MS-222 concentration is 100 mg/L, before and after keep-alive transportation, by measuring various physiological and biochemical indicators and blood indicator of tilapia and gold pomfret, it’s reveals that during anesthesia and keep-alive transportation, lactic acid and creatinine in the tilapia serum increase significantly, and blood sugar concentration is down after rising first.Tilapia and golden pompano the serum cortisol content in the soil conservation live there are significant changes before and after. PAGE PAGE VI By studying the effect of anesthetics concentration on tilapia survival time without water,