类固醇受体辅激活因子3在动脉粥样硬化中的作用-内科学专业毕业论文.docxVIP

目录2．2．2组织蛋白的提取，蛋白质的SDS．PAGE电泳及Westem 目录 2．2．2组织蛋白的提取，蛋白质的SDS．PAGE电泳及Westem blotting 分析 ．22 2．2．3细胞实验 23 2．2．4小鼠相关实验及方法 ．．25 第三章结果与分析 3 1 3．1 SRC．3缺陷小鼠动脉粥样硬化程度相对较轻 3l 3．1．1 SRC．3蛋白在动脉粥样硬化中高表达 ．31 3．1．2 APOE-／。SRC．30小鼠体重、心脏及肝脏重量较APOEo SRC．3岍小 鼠轻 32 3．1．3 APOE．／．SRC．3．／．小鼠主动脉粥样硬化斑块较APOE-／．SRC．3+／+ 小鼠少 33 3．1．4 APOE√’SRC．30小鼠肝脏脂肪堆积比APOE√‘SRC．3岍小鼠少 35 3．2 SRC．3显著增加巨噬细胞向泡沫细胞转化 36 3．2．1 SRC．3加速原代腹腔巨噬细胞转变成泡沫细胞 36 3．2．2敲低细胞内SRC．3能使巨噬细胞泡沫化减轻 ．37 3．3炎症反应在动脉粥样硬化的作用 38 3．3．1双敲小鼠循环血液中n师．c【和IL．1D细胞因子明显低于 APOE．／．小鼠 ．38 第四章讨论 ．40 参考文献 42 致谢 ．51 万方数据 ContentCoNTENT Content CoNTENT Abstract(In Chinese)．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．I Abstract(In English) ．．II Chapter I Introduction ．．1 1．1Nuclear Receptor ．．1 1．1．1 The structure of nuclear receptor ．1 1．1．2 The regulation of nuclear receptor ．．2 1．2 The SRC family and biological characteristics 3 1．2．1 Classify of SRC 3 1．2．2 The genetic of SRC ．．4 1．2．3 SRC modification 5 1．3 Biological characteristics of SRC一3 7 1．3．1 Relationship between SRC一3 and cancer 7 1．3．2 Relationship between SRC．3 and inflammation 一8 1．3．3 Relationship between SRC．3 and metabolism 8 1．4 Atherosclerosis．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．9 1．4．1 Lipid metabolism disorder． ． ． ． ．．10 1．4．2 Endothelium injury ．10 1．4．3 Inflammation 11 1．5 Aim of research ． ．14 Chapter II Materials and Methods．．． ．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．． ．．． ．．．．．．．．．．．．．．．．．．．．．．．1 5 2．1 Materials ． ． ． ．． 1 5 2．1．1 Mouse．cells and Bacteria ．．15 2．1．2Ⅺts ．．15 2．1．3 Enzymes and Antibodies 16 2．1．4 Reagents and supplies ．．16 2．1．4 Reagents and supplies 一17 2．2 Methods ．1 8 2．2．1 Molecular cloning ．18 万方数据 Content2．2．2 Content 2．2．2 Protein extraction and Western blotting ． ． ．． 22 2．2．3 Cells experiments．．．．．．．．．．．．．．．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．． ．．．．． ．．．．．．．．．．．．．．．．．．．．．．．．．．．．． 23 2．2．4 Mouse experiments．．．．．．．．．．．．．．