陆川猪皮肤组织转录组测序及毛色决定分子机制初步分析-动物遗传育种与繁殖专业毕业论文.docxVIP

织有8个基因表达显著下调，包括SIOOA7，SIOOA8，SIOOAl2，SIOOA9， 织有8个基因表达显著下调，包括SIOOA7，SIOOA8，SIOOAl2，SIOOA9， C14HlOorf99，ARGl，LOCMMPI。他们的log。FC均在4 和6之间，其基因的功能主要包括四个钙结合蛋白$100A7，$100A8， SIOOAl2和SIOOA9，一个基质金属蛋白酶(MMPl)，一个精氨酸酶 (ARGl)。对表达差异基因进行GO分析显示，在黑色皮肤中表达上调 的基因LOCLOCLOCKRT74和 LOC著富集(p=0．0008)于角蛋白，因而推测黑色皮肤 和白色皮肤所使用的角蛋白有所不同。差异表达基因KEGG Pathway 分析得知，白色皮肤因缺少TYRPl和TYRP2基因的表达造成代谢通路 无法合成真黑色素。此外，白色皮肤组织中还缺少基因黑色素体转移 相关基因TRPMl的表达。这可能是其肤色差异的最主要原因。 结论：白色毛皮合成真黑色素相关酶的基因TRYPl和TRYP2及黑 色素体转移相关的基因TRPMl均显著下调表达，这可能是导致毛色差 异的主要原因。 关键词：陆川猪；黑色皮肤；白色皮肤，差异表达基因；转录组测序 万方数据 A A PRELINⅡNARY STUDY 0N THE MOLECU『LAR Ⅳ匝CHANISM OF DETERMING COAT COLOUR OF LUCHUAN PIGS USING RNA—SEQ ABSTRACT To Study the molecular mechanism on the different formation of hair color in the lu—chuan pig with black and white coat colour．we utilizes the RNA-seq technology to sequence the mRNA which respectively extract from the black and white skin tissue in the same lu—chuan pig which growing in Guangxi Zhuang Autonomous Region and obtainand 3 1 1 7 1 1 36 reads．The overall uniq mapped to the reference genome (Sscrofal0．2 version)in pigs were accounted for 74．9％and 73．6％ separately．After analyzing the per base sequence quality,per sequence quality scores，per base sequence content，Sequence length distribution reads and the quality of mapping to the pig reference genome(Sscrofal 0．2)，the mRNA—seq sequencing data is reliable．The RNA-seq saturation analysis of covered gene reveals that the curve slope is close to zero illustrating that the data are close to saturation and with mgh credibility analysing gene expression patterns． The raw data are being quality controled，mapped，spliced and b：7jystematacially bioinformatics analysed．The analysis of differentially expressed genes between black and white skin tissue showing that 万方数据 comparing comparing to the white skin tissue，the black one having seven up regulating genes including TYRP 1，DCT，TRPM 1，LOC 1 KRT3 5 and two novel gen