灵芝三萜化合物的抗炎作用及分子机制初探-微生物学专业论文.docxVIP

内蒙古大学 内蒙古大学 硕士学位论文 检测相关指标的分泌量，验证ganodermanontriol对LPS／D—gaiN诱发小鼠肝炎的 保护作用。 结果如下：l、鹿角灵芝、赤芝、紫芝3种甲醇总提取物在100,jmL对细 胞增殖有一定的抑制作用，但在251．tg／mL、501ag／mL的剂量下对细胞没有抑制作 用。3种灵芝的甲醇总提取物在251ag／mL、50I_tg／mL浓度下对巨噬细胞NO、PGE2、 TNF．O【、IL．6、IL．1D释放量与LPS组相比较均显著降低。2、0【．1ignocericacid、 20一OH LucidericA，20·OH LucidericN、ganodermanontriol，Ganoderiol A、 Ganoderiol D、Ganoderiol F、Ergosterol peroxide、Ergosterol在5}tg／mL、lOgg／mL、 25I，tg／mL对细胞增殖均没有明显影响且NO、PGE2、TNF吨、IL-6、IL一1D分泌量 与LPS组相比较不同程度的降低，表明这9种化合物均有一定的抗炎作用。其 中20一OH lucidericN、ganodermanontriol、Ganoderiol A、Ergosterol peroxide四种 化合物抗炎作用更加显著。3、4种代表化合物均能抑制Myd88基因表达，且抑 制LPS诱发的NF—KB的激活，特别表现在抑制Ivd3．0【降解以及抑制Iv,B一0【和p65 磷酸化。4、LPS／D．galN诱发小鼠急性肝炎模型，ganodermanontriol给药组小鼠 肝脏组织病变较轻，且肝脏组织中AST、AIJ、TBIL、TNF．a、IL一6含量与 LPS／D．gaiN组相比显著下降，提示ganodermanontriol对LPS／D-gaiN诱发小鼠急 性肝炎有一定的保护作用。 关键词：灵芝提取物；三萜化合物；抗炎；分子机制；急性肝炎：保护 IV 万方数据 内蒙古大学 内蒙古大学 硕士学位论文 ANTI．n师LA^n僵ATORY EFFECT 0F‘I]肛 TRITERPE卜ⅢS ISOLATED FROM THE G么ⅣDD上识A伪三UC，D UA夕AND THE PRELIMINARY MOLECULAR卫皿CHANISMS ABSTRACT Inflammation is a physiological defense reaction of living tissues against harmful stimuli．However,prolonged inflammation can be harmful，contributing to the pathogenesis of many diseases．Nowadays more attention is paid on finding natural anti—inflammatory medicine with high efficiency and low toxicity．Ganoderma lucidum are used as traditional edible and medicinal materials in China．The triterpene，one major categoric of the bioactive ingredients from G lueidum．exert a variety of pharmacological activities．It is of great interest on the anti—inflammatory effect of G lucidum． The an—inflammatory effort in vitro was studied by lipopolysaccharide (LPS)．stimulated Ana一1 cell．At first，the study was focused on methanol extract of Ganoderma、G．1ucidum．karst and Gsininsis．We investigated the effect on cell proliferation of above three kind of methanol extract by MTS and detected the secretion ofNO、PGE2、TNF一值、IL一6、IL—l D．On the other hand，we investigated the anti—inflammatory effect of nine triterpene c