莲心总碱、阿霉素明胶微球的制备研究-药剂学专业论文.docxVIP

中南大学硕士学位论文 中南大学硕士学位论文 中文摘要 摘要 目的：制备莲心总碱(TAPN)、阿霉素(Adm)flfJ胶微球(Total alkaloids ofPlumula Nelumbinis and Adriamycin Gelatin Microspheres，TAPN-ADM·GE-MS)。 方法：采用乳化化学交联法制备TAPN。ADM．GE．MS。先采用单因素考查法 筛选影响其制备的主要因素，再采用均匀设计对其制备工艺进行优化，将得到的 数据用SPSSll．5和MATLAB7．0进行处理，确定其最优制备工艺，并进行验证。 用高效液相色谱法(HPLC)和紫外分光光度法(Uv)分别测定TAPN—ADM．GE-MS 中甲基莲心碱和阿霉素的含量及其包封率；通过激光粒度测定仪和扫描电子显微 镜对其粒径分布和表征进行分析。 结果：经过单因素考察和均匀设计实验，优选出TAPN．ADM．GE-MS的最优 制备工艺条件为：明胶浓度：22．5％、明胶与药物质量比：20、油水相体积比：17．5、 搅拌速度：900 r·min一、戊二醛用量：50 wE、Span．80用量：2％。按优化工艺参 数制得的TAPN．ADM．GE．MS载药微球呈圆球形，表面光滑，总载药量为：2．56％， 包封率为：66．44％，90％的微球分布在“10．100 ixm’之间。 结论：采用优化工艺条件制各的TAPN．ADM．GE．MS，制备工艺简单，载药 量较大、包封率较高、粒径分布相对集中，形态较好。为TAPN．ADM—GE—MS的 进一步研究打下了坚实的基础。 关键词莲心总碱，阿霉素，微球，动脉栓塞，制备工艺，均匀设计，高效 液相色谱法，紫外分光光度法 ABSTRACTObjective：To ABSTRACT Objective：To prepare Total alkaloids of plumula nelumbinis and Adriamycin Gelatin Microspheres(TAPN-ADM-GE—MS) Methods：The emulsification—crosslinking method was used to pre- pare the TAPN-ADM-GE—MS．At first，8 factors such as concentra-tion of gelatin solution were studied by single factor examination in order to choose those main factors．Then the optimum preparation formulation of the TAPN．．ADM-GE．．MS is achieved by employing the uniform design According to the results of the single factor examination and the optimum formulation was checked by experiment．The experimental data Was processed by SPSS 1 1．5 and Matlab7．0．The drug loading and embedding ratio of Neferine and Adriamycin about the TAPN-ADM—GE-MS Was detected by high performance liquid chromatography and ultraviolet spe- ctrophotomety respectively,the particle size distribution and superficial ．characterization of the TAPN-ADM．GE．MS were investigated by Mal— vern Zeta Nano-ZS and electronmicroscope espectively． Results：The optimum preparation formulation of the TAPN-ADM- GE．MS were as the follows：the gelatin solution(22．5％)，the weight ratio of the drug and loading material(20)，the volume ratio of oil and water (1 7．5)，the agitation speed(900r·min1)，the dosage of glutar