超临界CO2萃取白首乌中的C21甾苷.PDFVIP

※工艺技术 食品科学 2009, Vol. 30, No. 12 127 超临界CO2 萃取白首乌中的C21 甾苷 1 1,2, 2 吕志敏 ，田 鹭 * ，朱卫华 (1.盐城工学院化学与生物工程学院，江苏 盐城 224003 ；2.江苏大学化学化工学院，江苏 镇江 212013) 摘 要：目的：对白首乌中 C 5 21 甾苷超临界CO2 流体萃取工艺参数进行优化。方法：采用 L16(4 )正交试验，以萃 取压力(A) 、萃取温度(B) 、萃取时间(C) 、原料粒度(D) 、夹带剂量(E)作为考察因素，每个因素选四个水平，采 用 2- 去氧糖反应法测定各萃取条件下 C21 甾苷的相对含量，以波长590nm 处吸光度为判定依据，确定最优萃取条 件。结果：B 、D 、E 因素对 C 2 1 甾苷提取能力的影响最大，C 因素影响最小。结论：最优萃取条件为萃取压 力 30MPa 、萃取时间90min 、萃取温度55 ℃、原料粉碎粒度40 目、夹带剂量2.0ml/g 。 关键词：白首乌；C 21 甾苷；超临界CO 2 萃取；正交试验 Supercritical Carbon Dioxide Extraction of C21 Steroidal Glycosides from Cynanchum bungei Decne. Root . . 1 1,2, 2 LU Zhi-min ，TIAN Lu *，ZHU Wei-hua (1. College of Chemistry and Biological Engineering, Yancheng Institute of Technology, Yancheng 224003, China； 2. College of Chemistry and Chemical Enginerring, Jiangsu University, Zhenjiang 212013, China) Abstract ：Objective: To optimize the parameters of supercritical CO2 extraction of C21 seroidal glycosides from Cynanchum bungei Decne. root by orthogonal design. Methods: Five affecting factors such as extraction pressure, extraction temperature, extraction duration, particle size of material and 95% ethanol (entrainer) dosage were investigated by single factor test, and each factor was assigned with four levels for orthogonal design. Absorbance at 590 nm was taken to represent C21 seroidal glycoside content as the index to determine the optimal