不同烘烤条件下烟叶淀粉降解酶活性变化-河南农业科学.PDFVIP

DOI :10.15933/j.cnki .1004 -3268.2005.06.007 河南农业科学 1 2 ＊ 1 3 王怀珠,杨焕文 ,郭红英, 毛建书 (1., 550003;2., 650201; 3., 655000) :以烤烟品种红花大金元和K326 的中部叶为材料,研究了烘烤过程中烟叶淀粉酶和淀粉磷酸 化酶活 变化及烘烤条件对淀粉酶和淀粉磷酸化酶活 的影响。结果表明:烘烤过程中, 烟叶淀粉 酶活 出现2 次高峰,分别处于烘烤的变黄中期和定色前期;鲜烟叶中淀粉磷酸化酶活 较高, 随 着烘烤过程的推进, 至24 h 达第1 次高峰, 之后降低,48 h 时出现低谷, 随后红花大金元于60 h 达 第2 次高峰,而K326 于72 h 达第2 次高峰。在整 个烘烤过程中,采用低温低湿变黄,慢速升温定 色的烘烤条件, 烟叶内淀粉酶和淀粉磷酸化酶活 较高,有利于烟叶淀粉的降解。 :烤烟;烘烤条件;淀粉降解;酶 :S572 　　:A 　　:1004-3268(2005)06-0030-04 The Activity Changes of Starch Degradation En ymes in Tobacco Leaves under Different Curing Condition 1 2 ＊ 1 3 WANG Huai-hu ,YANG Huan-wen ,GUO Hong-ying ,MAO Jian-shu (1.Gui hou T obacco Science Research Institute, Guiyang 550003, China; 2.College of Tobacco of Yunnan Agricultural University, Kunming 650201, China; 3.Qujing Tobacco Company of Yunnan Province, Qujing 655000, China ) Abstract:The effects of curing condition on the activity of amy lase and starch phosphorylase and the changes of the activity of amylase and starch phosphorylase in tobacco leaves during flue-curing w ere studied by using the flue-cured tobacco cv.HD and K326 as experimental material.The results indi- cated that tw o peaks of amylase activity appeared at middle stage of yellow ing and early stage of flxing- color during curing, respectively .The activity of starch phosphorylase was relatively higher in fresh leaves.With the proceeding of curing, the activity of starch phosphory lase reached its first peak at the 24th hour, follow ed by a valley at the 48th hour, and then,that of HD reached the