沙门菌分离株耐药谱及鼠伤寒沙门菌多重耐药机制的研究-营养与食品卫生学专业论文.docxVIP

皇曼茎塞墨堕墼墅生：垒堡圭鎏苎 皇曼茎塞墨堕墼墅生：垒堡圭鎏苎 鲨盆壁坌查楚墼蔓堂墨墼堑鲞鲨!!整墨墨壁罂璺塑!!翌噬 Charater主z8tion of Antimicrobial Resistant Salmonella and Molecular Mechanisms of Multiple AntimicrobialResistantce in Abstract Thee methods were used to identify Salmonella．52 Salmonella isolates of total 55 suspect Salmonella isolates,which isolated from retail chicken in 8 provinces of China，were 100％identified by API 20E system，polymer冀se chain reaction and serological tesL The first two memods was more accurate than serological test，but they can only provide the identification at genus level，the serological test can differentiate the Salmonella with species． rifty one strains of identified Salmonella isolates above were examinated for antimicrobial susceptibility with 30 kinds of antimierobial agents by E-test method．All of the strains were resist馥nt to at least three antimicrobial agent． Eight strains(15。7钧were resistant to three antimierobial agents, thirteen(2s．5％)strains to four antimierobial agents，nine 07．6％)strains to five antimierobial agents，twelve strains翁35％)to from six to nine antimierobiai agents and nine strailLS(17．6％1 to ten antimierobial agents．AII of the 51 strains welqe resistent to vancomycin and erythromycin and mezlociHin,and for other antimierobial agents,the strains showed different percent of resistenee． Three strains were resistant to gentamicin，two strains were resistant to amikaein and trimethoprin， and one stems was resistant to trimethoprim／sulfamethoxazele．强e important thing is量Salmonella 氆pMmurium isolate,which exhibiting the similar antimicrobial resistance profde 舾serovar thphimurium DTl04．was isollted in ret矗il chicken from Fujian province，and the ioslated strains was resistant to fifteen antimicrobial agents． The MICs of three Salmonella(yphlmurium isolates X2,XT,X11 to eiprofloxaein were above 32转g／ml，0．38t嘲g／ml and O．023pg／ml，respectively．Due to mutations of fiuoroquinoione resistance-determining regions(QRDRs)were main cause of the resistenee of Salmonella《yp