B淋巴细胞介导的体液免疫应答.ppt

第十三章 B淋巴细胞介导的体液免疫应答 Antigen recognition of B cells B cell recognizes and binds an antigen in its free, unprocessed form through BCR. No MHC restriction. Double signals for B cells activation Antigen signal: recognition of antigen by the BCR Igα/Igβ(CD79a/CD79b): transmit antigen stimulus signal Co-receptors: CD19/CD21/CD81 accessory signal CD40 CD40L Interaction between TH and B cells TH cells provide accessory signal （CD40/CD40L） and cytokines (Th1 IL-2、IFN-γ、Th2 IL-4、5、6)for B cells activation and differentiation. B cells provide antigen signal （peptide-MHCⅡ complex）and costimulatory signal （B7/CD28） for TH cells activation. Proliferation and terminal differentiation of B cells Activated CD4+Th secrete cytokines which act on B cells Formation of plasma cells Synthesize and secrete specific Ab Most transfer to bone marrow and generate Ab continuously Don’t express BCR and MHCⅡ molecule, can’t react to Ag and lose the ability to react with Th TI antigen TI-1 antigen TI-2 antigen TI-1抗原 又被称为B细胞丝裂原，如LPS，成熟或不成熟的B细胞均可被激活。高浓度时经丝裂原受体与B细胞结合诱导多克隆B细胞增殖和分化；低浓度时激活抗原特异性B细胞。 激活初始B细胞无需Th细胞辅助，诱导产生低亲和力的IgM，单独不足以诱导Ig类别转换、抗体亲和力成熟及记忆性B细胞形成。 Primary response Most of the antigen is processed by dentritic cells. Develop in 4-5 days after antigen introduction, 5-10 days required to reach peak serum antibody concentration. Achieve low antibody concentration. Duration is short. The antibodies initially produced are predominantly IgM with low antigen affinities. Secondary response Meromy B cells serve as the principal APCs. Activated at very low concentrations of antigen. Shorter lag peroid. Achieve high affinity and high concentration of antibody. Duration is long. IgG tends to predominate. （四）记忆性B细胞的产生 生发中心中存活下来的B细胞，或分化发育成浆细胞，或成为记忆性细胞离开生发中心进入血液参与再循环； 记忆性B细胞不产生Ig，但再次与同一抗原相遇时可迅速活化，产生大量抗原特异的Ig。 Antibody: neutralization, opsonization, complement activation and ADCC. Memory B cells Effector 五、B细胞的效应 抗体的效应 记忆性B细胞的效应 第二节 B细胞对TI抗原的免疫应答 Chapter 2 B cell response