硫酸盐还原菌腐蚀的微生物防治研究-应用化学专业论文.docxVIP

华中科技大学硕士学位论文摘要 华中科技大学硕士学位论文 摘要 (硫酸盐还原菌(SRB)是导致微生物腐蚀的主要菌属之一，绘工业生产和环境 带来日懿严重的影响，丽使人们越来越重视研究对它的防治方法。目前通常采用的 防治方法是投加杀菌剂，对于从生物学角度去寻找新的方法则研究罄少。】 本论文以探索微生物防治SRB腐蚀为出发点，首先根据SRB的生长特点，选择 脱氮硫秆菌(T．denitrificans)作为防治SRB腐蚀的菌源。从土壤中分离和提纯出该 菌，并通过研究其培养特性，和借助透射电镜观察该菌株的形貌，对该菌株的种瘸 进行了签定。研究发璃该菌株呈秆狄，荜个、戒对葳短链状捧捌，具有筚裉辙生鞭 毛，无芽恣，兼瞧厌气，专往无梳亿箍垂券型，确定箕属予聪氮硫杆菌；脱氮硫抒 蓥生长姆牲楚生长缓熳，生长过稷孛产生至{+，壤蓁蒸戆p珏黪绦；其最佳生长舞l 为7。o，秘SRB生长的pH傻楣返，为疆者混合壤莠提供可行性依撼。(脱氮磙棵萤藏 量少时，腐蚀比无菌空白培券介质小，随着菌量的增加，腐蚀也增加。土 ／ 本论文研究了T．denitrificans与SRB共同培养时，对SRB生长和代谢的影响。 测定了T．denitrificans与杀菌剂联会使用时对杀死SRB的影响。利用失重法、电位跟 踪、极化曲线、电化学交流阻抗、电子探针、扫描探针显微镜等方法，对脱氮硫杆 菌防治SRB腐蚀中的腐蚀产物的组成，生物膜的形貌以及电化学行为进行了研究。 ， 、研究表明T．denitrificans可将SRB代谢产生的腐蚀性硫化物转变为硫酸根离子，从而 使生物膜致密雨均匀，等致电荷传质电阻增加，腐蚀电流减小丽降低了SRB瘸蚀。0 关键漏；硫酸箍还原蔻?微生耱藤落i残氮硫枰藏；微生锯茨治方法天电亿学隧蕊， 电予探锋：扫接探针显微镜 华中科技大学硕士学位论文A8STRACT 华中科技大学硕士学位论文 A8STRACT Corrosion caused by Sulfate Reducing Bacteria(SItB)is one of the most common forms of microbiologically influenced corrosion(MIC)in the oilfield．The corrosion caused by SRB，showing severe effect on industry manufacture and environment，gets more and more aaenfion to be investigated for its controlling。At present，bactericides are probably the most common method of anti—biocorrosion．However,less attention has been paid to the microbi0109ical control of SRB induced corrosion． In this thesis．the microbiological control of SRB has been studied．Thiobacillus denitrificans was selected to control the corrosion caused by S}国according to the corrosion characteristic of SRB．A s打ain of T．denitrificans isolated from the soll which dispose in solitude or gemination has one flagellum and no spore．The growth curve shows that T．denitfificans slowly grow and H+is produced during the metabolism．The optimal pH for T．denitrifieans growth is 7．0．being close to that of SRB．This indicates that t denitrititans could coexist with SRB and may be used to control the corrosion caused by SRB。11沁corrosion rate was less than that of tlle sterile culture when culture medium Was inoculated with a feW T．denitrificans．