雷公藤单体抑制胶质瘤细胞与血管生成的实验研究-神经外科学专业论文.docxVIP

中文摘要驴j互591 中文摘要 驴j互591 51 第一部分 目的研究雷公藤单体对胶质瘤细胞及血管内皮细胞株的体外抑制作用 方法通过MTT法测定四种雷公藤单体对胶质瘤细胞株SHG44、C6、U251 及血管内皮细胞株ECV的体外抑制作用。 ／结果发现雷公藤单体中三萜类红素等对胶质瘤细胞有一定的抑制作用而且 对h管内皮细胞有明显的抑制作用。而雷公藤二萜类单体雷公藤甲素及LLDT— 10对胶质瘤细胞有极明显的抑制作用，但对血管内皮细胞无作用。，， 结论 红素对血管内皮细胞有明显抑制作用而二萜类中的雷公藤甲素等对 胶质瘤细胞有明显的抑制作用。体外实验为今后体内实验提供了依据。 “关键词l：雷公藤红素重坌壁里塞内皮细胞些要塑{ 第二部分 雷公藤红素抑制血管生成的实验研究 目的：探讨雷公蘑红素对血管生成的抑制作用。 方法：采用Mrr法测定雷公藤红素对血管内皮细胞株ECV增殖的影响， 观察雷公藤红素对ECV迁移实验和小管形成实验的作用。以及对鸡胚尿囊膜血 管生成的影响。血管生成抑制的体内实验采用Matrigel plug实验。 I结果：雷公藤红素明显抑制ECV的体外增殖，ICso为1．33 u g／ml ，而且 可抑制ECV的迁移和小管形成，并呈明显的剂量依赖性。同时也抑制鸡胚尿囊 膜血管生成作用和Matrigel plug中的血管新生。F 结论：雷公藤红素具有明显抑制血管生成的作用，有可能成为极具前途的 血管生成抑制剂。 【关键词J血管生成 雷公藤红素内皮细胞 ＼_／7 √＼／ 璺型塑!垒蜜婴堕!墅垡连翌型墅型苎竺型鲤墼哩墅堂!塑曳连竺!煎堕垒墼Abstract 璺型塑!垒蜜婴堕!墅垡连翌型墅型苎竺型鲤墼哩墅堂!塑曳连竺!煎堕垒墼 Abstract First part Several monomes from Tripterygium wilfordii inhibite proliferation of glioma cells and vascular enththelial cells砌vitro 0bjeetive：The current study was designed to investigate inhibitive effect of several nlonomes of Tripterygium wilfordii On the proliferation of glioma cells and enththelial cells of vessels(Ecv)． Methods：T．he effect of four monomes#ore Tripterygium wilfordii on the proliferation of glioma cells SHG44、C6、U251 and ECV in vitro was examined using MTT assay． Result：The proliferation of ECV Was inhibited by tripterine，which belong to triterpenes．So WaS glioma cells．Triptolide and LLDT-10 treatment resulted in significantly inhibition of glioma cells except ECV． Conclusion：These findings suggest tripterine inhibited the proliferation ofECV Triptolide had significantly inhibition of glioma cell．These data from experiment in vitro provide foundation for future experiment in vivo．． IKey word]Tripterine Triptolide endothefial cell glioma Second part Experimental studies on inhibition of neovascularization by Tripterine 0bjective：To investigate inhibition ofneovasculmization by Tripterine． Method：The effect of tripterine on the proliferation of ECV in vitro WaS examined using MTT assay．We analyzed the effect of Tripterine on endo