兰州地区小儿病毒性腹泻的临床流行病学研究-儿内科学专业论文.docxVIP

!卅I火学硕士学位沧文结论：兰州地区婴幼儿病毒性腹泻的病原复杂，轮状病毒仍是最主要病原， !卅I火学硕士学位沧文 结论：兰州地区婴幼儿病毒性腹泻的病原复杂，轮状病毒仍是最主要病原， 本年度轮状病毒的主要流行株为P[a]C2，与往年明显不同，杯状病毒G GII一4型 和星状病毒l型分别为主要的流行株，不同病原混合感染比例较大，值得重视。 关键词：婴幼儿腹泻轮状病毒杯状病毒星状病毒肠道腺病毒 II 兰州大学硕士学位论文ABSTRACT 兰州大学硕士学位论文 ABSTRACT The Etiological Study of Viral Diarrhea with Children in Lanzhou objective：Acute gastroenteritis is common health problem in children worldwide．It has been confirmed that socio—economic improvements in water and sanitation may not significantly reduce the incidence of viral diarrhea．Furthermore，it was absent specific medicine in clinic．We study molecular epidemiology of four major etiological viruses among children with acute diarrhea in Lanzhou，Gansu province China from July2004 throu曲June2005．Our purpose is to accumulate datum for prevent and control Viral Diarrhea among Children in the region and to provide proof for develop correlate vaccine also． Methods：Stool specimens were collected from all inpatients less than 5 years old with acute diarrhea admitted in Department of Pediatrics，the First Hospital， Lanzhou University from July2004 through June2005．Dako IDEnTM kits were used for detection of rotavirus，adenovims and astrovirus，and calicivirus detection by both reverse transcription-polymerase chain reactions(RT-PCR)and Enzyme—linked immunosolbant assay O!LISA)．Further strain characterization of rotavirus and astrovims were done with RT-PCR．Some PCR amplicons of calicivims and astrovirus were cloned and sequenced．Phylogenetic tree was constmcied for strain charactefization． Results：A total of 400 stool samples were collected and tested．Rotavirus was present in 47．3％of cases，calicivims in 15．5％，astrovirus in 9．5％and adenoviruses in 7_3％．Mixed infections with multiple enteric viruses were present in 1 3．5％of all samples．Among 189 rotavims positives，serotype G2(34．4％)was the predominant strain followed by G3(32．8％)，GI(I．1％)and mixed—G infection(5．8％)．25．9％of strains remained to be non—typeable．P genotyping showed P[41(45％)wa