磷脂酰胆碱富集及热原去除的研究-粮食、油脂及植物蛋白工程专业论文.docxVIP

AbstractAbstract Abstract Abstract Phospholipids is a mixture of phosphorus lipid，with different physicochemical and functional characteristics．It has been widely used as all emulsifier in the food and pharmaceuticals industries．，nle aim of the present work Was to establish an optimized route for phosphatidvrcholine enrichment by the ethanol heating extraction and frozen purification method,and removal of pyrogen by active carbon technologies．，nle new products call be inj ected were produced． nle soybearl lecithin products with PC content in 72．85％and PE content in 5-1 O％were extracted from soybcan powder phospholipids．111e effects of ethanol concentration, ethanol／1ecithin ratio，extraction temperature，extraction time，freezing temperature and freezing time on product yield and the PC and PE content were investigated．Hi曲yield Was expected，as well as the PC content and PE content of the product ranged from 72％to 85％ and from 5％to 1 0％，respectively．nle results showed that the superior conditions were 95％ ethanol concentration,ethanol／lecithin ratio 4：1，extraction temperature 60C，extraction time 45min,freezing temperature一1 5C，freezing time 30min．Based on the results of factorial tests， response surface methodology(RSM)Was utilized to further investigate the effects of ethanol／lecithin ratio，extraction temperature，freezing temperature and freezing time．Under the optimized conditions，i．e．ethanol／lecithin ratio 4．50：1，extraction temperature 62．75℃， freezing temperature-13．96℃，freezing time 30．00min，the product yield was 24．17％，PC content WaS 73．89％，PE content Was 1 0．00％． T11e feasibility of the bacterial endotoxin test by Tachypleus Amebocyte Lysate(TAL) for soybcan lecithin products Was studied．111e interfering effect WaS not shown when the soybearl lecithin solution丽tll the concentration of l Omg／mL Was diluted to 3 folds．TAL call determine the number of units of endotoxin in phospholipids．Using limulus bacterial endotoxin test to detect the endotoxin level of inj