磷胁迫下油菜幼苗叶片差异蛋白表达的初步分析-河南农业科学.PDF

DOI :10.15933/ki.1004 -3268.2011.08.035 　河南农业科学,2011,40(8):89-91, 112 　Journal of Henan gricultural Sciences ＊ ＊ ＊＊ 余永芳 , 赵丹丹 , 李玉琴, 牛银银, 田保明, 臧　新 ( , 450001) :以油菜品种中油821为材料,利用双向电泳技术研究足磷和缺磷条件下油菜幼苗叶片差 表 达的蛋白质,进而为揭示油菜耐低磷胁迫的分子机制奠定基础。结果表明:经电泳、PDQuest 分析后, 缺磷处理下差 显著的蛋白质点有38 个, 19 个表达量显著上调, 19 个显著下调。磷胁迫下蛋白表达 量的差 说明逆境下植物体可通过多种蛋白的协调作用来适应外界环境的变化。 :油菜;磷胁迫;双向电泳;蛋白质组学;差 蛋白表达 :S565.4 　　: 　　:1004-3268(2011)08-0089-04 Preliminary nalysis of Differential Proteins of B rassica nap us Leaves under Phosphorous Deficiency ＊ ＊ ＊＊ YU Yong-fang ,ZH O Dan-dan ,LI Yu-qin,NIU Yin-yin, TI N Bao-ming,Z NG Xin (Bioengineering Department,Zhengzhou University,Zhengzhou 450001, China) Abstract:The proteome changes in the seedling leaves of Zhongyou 821 was investigated by 2- DE, which could lay the foundation for the molecule mechanism of the rapeseed endured low phosphor.T he results showed that, analysed by 2-DE, PDQuest software, the remarkable differ- ence points of the young plants treated with no enough phosphoric were 38, and 19 proteins were up-regulated, and the other 19 points were down-regulated, which revealed that many proteins invovled in the physiological process under phosphorous deficency . Key words:Rapeseed;Phosphorus deficiency;Two-dimensional gel electrophoresis;Proteomics; Differential protein expression 　　 ,, [4] , 。 ;, ,6。 [5] 、, , ,、 [1] 。 、、。 , 。 821, ,, ,, [2] 。,, 。 。 1　 。, 1.