电磁辐射对大鼠心肌细胞Bcl-2和Bax表达的影响-中国辐射卫生.PDFVIP

DO I :10.13491/j .cnki .issn.1004 -714x.2010.02.056 ·132 · 20 10 6 19 2　ChinJRadiolHealth, Jun2010, Vol19, No2　 【】 Bcl-2 Bax 1 2 1 2 1 1 1 　, , ,, , , :Q64　:A　:1004-714 X(2010 )02-0132-0 3 　　【】　　 3 Bc-l2 Bax, 。　180 Wistar (C)、S (S)、X (X)、 (E), 6 h、1 d、3d、7 d、14 d、28 d, 、 Bc-l2 Bax, , 3 Bcl-2 Bax 。　3 6h～7 d, , , , , , ;, , Bax Bcl-2 (P 0.05 ), Bax Bcl-2 。 14 ～2 8 d, 14 ～2 8d Bcl-2 Bax 6 h～7 d, Bcl-2 Bax, 。 6 ～ 12 。　, , Bax Bcl-2 , , X S。 【】　;;Bcl-2;Bax AStudyoftheExpressionofBcl-2 andBaxinRatHeartsInducedbyElectromagneticRadiations.DOUYan, WANGDe -wen, ZHANGMin-feng, eta.l1 The17 5 thhospitalofPLA, Zhangzhou363000 China;2.InstituteofRadiationMed-i cin,eAcademyofMilitaryMedicalScience,sBeijing100850 China. 【Abstract】　Objective　 TostudycontrastlythevariationoftheexpressionofBcl-2 andBaxproteininratheartsin- ducedbythreekindsofBandElectromagneticRadiations[ X-band、S-bandandElectromagneticpluse(EMP)] andinvest-i gatethepossiblemechanismofapoptosis.Methods　180 healthyWistarratswererandomlydividedintofourgroups:acontrol group(n=36)andthreeexperimentalgroups(n=48)respectivelytreatedwithX-band、S-bandhighpowermicrowaveand EMP.Atdifferenttmiepoints(6 hour,s1、3、7、14 、28 day,s6 month,s12 months), theratswerekilledafterirradiated.We observedthepathologicalchange,sexaminedtheexpressionofBc-l2 andBaxproteinbyusingimmunohistochemicaltechnique andstudytheruleofchangequantitativelybyusingmiagineanalysistechnique.Results　Theinjuryofratheartswasmoreand moreseriousat6 hours-7 days:cardiomyofilamentswerederanged;Glycogenweredicreased;Purkinjecellslysi;smartixswel-l ingandexudativeserous.TheexpressionofBaxandBcl-2 proteinwasbothstrongerthanthecon