文献标识码-现代食品科技.PDF

现代食品科技 Modern Food Science and Technology 2013, Vol.29, No.1 盐酸克伦特罗与莱克多巴胺联检速测卡的研制 罗奕铭 （广州瑞森生物科技有限公司，广东广州 511400） 摘要：本文研究建立了一种检测盐酸克伦特罗与莱克多巴胺联检速测卡的方法。应用胶体金免疫层析技术，采用柠檬酸钠还原 法制备胶体金颗粒，标记盐酸克伦特罗与莱克多巴胺单克隆抗体并喷于玻璃纤维上，盐酸克伦特罗与莱克多巴胺偶联OVA 抗原和羊 抗鼠 IgG 分别结合于硝酸纤维膜上，依次将样品垫、胶体金垫、硝酸纤维素膜和吸水纸组装切割成盐酸克伦特罗与莱克多巴胺联检 速测卡。试验结果表明，该联检速测卡的灵敏度分别为CLEN 3 ng/mL、RAC 5 ng/mL，检测时间为3 min，批内和批间重复性为100%， 假阳性率和假阴性率均为0 。 关键词：盐酸克伦特罗；莱克多巴胺；胶体金免疫层析；联检速测卡 文章篇号：1673-9078(2013)1-197-200 Research of Synchronizating Rapid Detection with Clenbuterol and Ractopamine LUO Yi-ming (Guangzhou Ruisen Biotechnology Co., Ltd, Guangzhou 511400, China) Abstract: This study established a method of synchronizating rapid detection with clenbuterol and ractopamine by using colloidal gold immunochromatography and sodium citrate reduction for preparation of colloidal gold particles . To mark clenbuterol and ractopamine monoclonal antibody, and sprayed on glass fiber, the antigen of clenbuterol and ractopamine coupled OVA and sheep anti-mouse IgG were combined with nitrocellulose membrane respectively. Then, the sample pad, colloidal gold pad, nitrocellulose membrane and absorbent paper were assembled and cut into Articles. The results showed that sensitivity of the rapid detection article was 3 ng/mL for CLEN and 5 ng/mL for RAC .The detection time was 3 min and intra-and inter-reproducibility was 100% . False positive rate and false negative rates were 0. Key words: clenbuterol; ractopamine; colloidal gold immunochromatography; rapid detection card 进入 21 世纪以来，食品安全问题受到越来越多 在硝酸纤维素膜上预先固定好特异性抗原(或抗体)和 人的重视，我国多起瘦肉精中毒事件的频繁发生，引 抗体作为 2 种捕获剂，分别设定为检测线(T 线)