流式细胞技术荧光抗体.pptVIP

Fluorochrome Properties Desirable properties for fluorochromes: High relative brightness Narrow emission spectrum (low spectral overlap in combination) Easily conjugated (for immunophenotyping) Fluorochromes can be characterized by: Type of molecule Excitation and emission wavelengths Relative brightness Fluorochrome Molecule Types Some Common Fluorochromes Small Organic Fluorochromes Protein Fluorochromes Tandem Dye Fluorochromes Nanocrystal Fluorochromes Excitation and Emission Excitation wavelengths determine lasers that can excite the fluorochrome. Emission wavelengths determine filters