桑树离体培养与多倍体诱导研究-作物遗传育种专业论文.docxVIP

II II 二倍体植株的染色体数目为2n=2x=28。桑树四倍体和二倍体的气孔数目及保卫细胞 大小之间的差异显著。 关键词：桑树；离体培养；秋水仙素；化学诱变；多倍体 III III Study on the In Vitro Culture and Polyploid Induction of Mulberry (Morus alba) Zhu Hong (College of Agriculture, Guangxi University, Nanning 530004, China) Abstract Mulberry fine varieties Guisangyou 62 and Guisangyou 12 were taken as test materials to study in vitro culture and polyploid induction of mulberry. For the study of in vitro culture, the effects of different sterilization methods on explants sterilization, different basic mediums and hormone treatments on asepsis bud multiplication, and different hormone combinations on asepsis plantlets root induction were explored. Therefore, the suitable explants sterilization method, bud multiplication medium and root induction medium were selected, and the technology system of in vitro culture of Guisangyou 62 and Guisangyou 12 was established, in which it could provide technical reference for industrialized breeding of Guisangyou 62 and Guisangyou 12. For the study of polyploid induction, effects of different colchicine concentrations treating the same time and one colchicine concentration treating different time on polyploid induction were studied, and morphological identification and cytological identification were conducted on the variant plants obtained. The main results were as follows: The best sterilant combination for the seed explant sterilization was 0.1% HgCl2 14min+10%NaClO 12min. 75% Ethanol was not suitable for the sterilization of mulberry seeds. MS medium without GA3 was better for plantlet induction of mulberry seeds. In the comparison test of three auxins(IBA, NAA and IAA), the best auxin for asepsis bud multiplication was IAA; in the comparison test of five basic mediums(MS, B5, N6, Miller and White), the best basic medium for asepsis bud multiplication was MS medium. PAGE PAGE VI The best medium combination for asepsis bud multiplication of mulberry was MS+IAA 0.2mg/L+6-BA 2.0mg/L+CPPU 0.8mg/L+sucrose