滑桃树愈伤组织与内生真菌共培养的代谢产物研究-厦门大学学报.PDFVIP

49 3 厦门大学学报( 自然科学版) Vol.49 No. 3 2010 5 Journal of Xiamen University (Natural Science) ay 2010 吴 欣,鲁春华* (, 361005) : ( Fusarium sp. WXE) , . , . 3 , (16A)-17-hydroxy-ent-atisan-19-oic acid methyl ester ( 1), ( 16A)-16, 17-dihydroxy-ent-atisan-19-oic acid methyl ester ( 2) B-(3). : ; ;; :Q 945; Q 939.9 : A :0438-0479( 2010)03-0406-04 254 , (T S); GF ;, (200~ 300 ), ; RP-18 erck ;Sephadex LH- [ 1-3] ,, . 20 Amersham Pharmacia . (Trew ia nudif lora L.), 1.2 1.2. 1 [4-6] . , 30 [7] , min , , 75%() , 1 min, 0. 035 g/mL NaClO 5 min, 0. 002 g/ [8] mL HgCl2 8 min . , ( Fusar ium sp. WXE) , ,4 mg/L , . , 26 e . 1.2. 2 1 1~ 2 cm [9] , S( urashige and Skoog) 1. 1 1. 0 mg/L 2, 4-( 2, 4-D) 1. 1. 1 1.0 mg/L (NAA). 2~ 3 , , 4 . ., 1. 1. 2 .0.2 mg/ L (KT), BioESI-Q-TOF(Bruke) ;Bruker DRX 500