联合运用CNTF与IGF促神经再生作用的实验研究外科学专业论文.docxVIP

PAGE PAGE 10 4 结论 1 外源性胰岛素样生长因子（IGF）与睫状神经营养因子（CNTF） 局部运 用均有促神经再生的作用。 2 胰岛素样生长因子（IGF）、睫状神经营养因子（CNTF）联合运用对周围 神经损伤后再生有协同作用。 3 同等剂量下胰岛素样生长因子（IGF）、睫状神经营养因子（CNTF）促神 经生长作用早期无显著性差异。 关键词：胰岛素样生长因子 睫状神经营养因子 周围神经再生 神经营养 因子 The experimental study to promote nerve regeneration by joint use between CNTF and IGF in rats Abstract 1 Objective:The purpose of this study is locally use exogenous nerve growth factor ciliary neurotrophic factor (CNTF), insulin-like growth factor (IGF) and joint use of them, comparing the function of Peripheral nerve regeneration after injury each other,to find a better way to promote nerve regeneration . Method : A total of 48 wistar rats were randomly divided into four groups that group A IGF + CNTF, Group B IGF, Group C CNTF, group D blank control group. We established a model of sciatic nerve injury and a small room for nerver regeneration, and injected different drugs into the small room ，group A IGF + CNTF + chitosan , group B IGF + chitosan , group C CNTF + chitosan and group D chitosan + saline. Then after established model we measured and observed the sciatic nerve index (SFI) in 2, 4, 6, 8 weeks, taking Speed and amplitude of operated side sciatic nerve action potential in 4, 8 weeks , and finally killed all of the rats after 8 weeks taking out of regenerated nerve in small room for histological observed (light microscopy, electron microscopy). Using image analysis software Image-proplus to measure the average diameter of axons, the number of regenerated axons and the percentage area ratio in per unit area. All the results of the observation target was analyzed the main effect of IGF and CNTF and interactive effects by variance analysis of factorial design, IGF group and CNTF group was analyzed by two sample t-test, the test Standard is α = 0.05. Results1 Sciatic nerve index (SFI) :the variance analysis of factorial design for SFI proved that IGF and CNTF main effects and interactions were both significant, P0.05,Combined with t