高效液相色谱光化学在线衍生荧光法检测鸡肉中16种磺胺类药物残留.pdf

※安全检测 食品科学 2015, Vol.36, No.22 165 高效液相色谱光化学在线衍生荧光法检测鸡肉中 16 种磺胺类药物残留 1,2 1,2 1 1 1,* 1,* 许 旭 ，耿丹丹 ，迟晓峰，董 琦 ，胡风祖 ，肖远灿 （1.中国科学院西北高原生物研究所，青海 西宁 810008 ；2. 中国科学院大学生命科学院，北京 100049 ） 摘  要：采用高效液相色谱-光化学柱后在线衍生-荧光检测法检测鸡肉中16 种磺胺类药物残留量。鸡肉样品经过乙 腈提取并脱脂净化，色谱柱分离，光化学在线衍生并直接用荧光检测器检测。流动相为0.3%的冰乙酸和甲醇溶液梯 度洗脱，流速0.7 mL/min 、柱温36 ℃，激发波长320 nm、发射波长450 nm ，16 种磺胺类药物得到良好分离。16 种 R % % 磺胺药物在0.13～67.89 µg/mL质量度范围内，线性良好（ ≥0.994 0 ），回收率在61.2 ～106.6 之间，相对标准 偏差为0.3%～13.9%，检出限为1.0～67.4 µg/kg 。该方法灵敏、准确、快速，可满足鸡肉中磺胺类残留的检测。 关键词：鸡肉；磺胺类药物；光化学在线衍生；高效液相色谱-荧光检测 Determination of 16 Sulfonamide Residues in Chicken by High Performance Liquid Chromatography with Online Photochemical Derivatization and Fluorescence Detection 1,2 1,2 1 1 1,* 1,* XU Xu , GENG Dandan , CHI Xiaofeng , DONG Qi , HU Fengzu , XIAO Yuancan (1. Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810008, China; 2. College of Life Science, University of Chinese Academy of Science, Beijing 100049, China) Abstract: A high performance liquid chromatography (HPLC) with online postcolumn photochemical derivatization and fluorescence detection (FLD) method was established for the determination of 16 sulfonamide residues in chicken. Chicken samples were extracted with acetonitrile and the extract was defatted, separated by column chromatography, treated by on- line photochemical derivatization and detected using a fluorescence detector. The mobile phase was a mixture of 0.3% glacial