应用纳米金探针检测hsv-2的目视化基因芯片资料精.pdfVIP

第 21 卷 3 期 中 国 病 毒 学 21 （3 ）：294-297 2006 年 5 月 VIROLOGICA SINICA May 2006 应用纳米金探针检测 HSV-2 的目视化基因芯片* 1 1** 2 1 1 刘明贵 ，王业富 ，隋 放 ，万志香 ，高文娟 （1.武汉大学生命科学学院 病毒学国家重点实验室, 湖北武汉 430072 ； 2.辽阳市中心医院, 辽宁辽阳 111000 ） A Visual Gene Array for the Detection of HSV-2 Based on Nanogold Probes 1 1** 2 1 1 LIU Ming-gui ，WANG Ye-fu ，SUI Fang ，WAN Zhi-xiang ，GAO Wen-jun (1. State key Lab of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China ；2. Liaoyang Center Hospital, Liaoyang 111000, China ) Abstract ：A visual HSV-2 detection gene array was developed based on nanogold probes 。The specific primer and probe of HSV-2 were designed based on the highly conservative region of HSV-2 DNA polymerase gene and the HSV-2 PCR amplicon were labeled by biotin through PCR reaction ．Amino-modified probes were spotted on an activated glass surface on which hybridized with the PCR amplicon ．Subsequently, the streptavidin-nanogold was added to form the amplification system of the biotin-streptavidin-nanogold complex ．At last, the step of silver staining amplified the signal of hybridization, Therefore it was easy to detect the HSV-2 by naked eyes ．As little as 100fmol/L of HSV-2 amplicon could be detected on the array, The HSV-2 detection gene array can detect the HSV-2 virus infection with high sensitivity and low cost. Key words ：Gene array; HSV-2; Nanogold; Biotin-streptavidin; Sensitivity 摘要：本实验建立了一种应用金标链霉亲和素探针的目视化高灵敏度检测单纯疱疹病毒 2