两种猪肠道冠状病毒诊断方法的建立和国内新分离毒株主要结构蛋白基因克隆与序列分析.pdf

两种猪肠道冠状病毒诊断方法的建立和国内新分离毒株主要结构蛋白基因克隆与序列分析.pdf

F ARSTRAC closedwithNterminalofS is thesizeabout DNA was gene,and 922bp.Thefragment clonedsite intoPET-32avectorbetween V specially theEcoRandSalI site.The cleavage Pet一32a recombinantedwasverifiedrestriction plasmid by endonucleaseand analysis nucleotide itwastransformed intoE.colistrain sequencing.Then RosettaforSgene resultofSDS.PAGE indicatedthatthe of expression.The recombinantTGEVS production its about reached 5-8 hoursafter at28。Candthe can peak take inducing expressionprotein 25%ofthe about total wasidentified protein.The Westernblottest. expressionproduct by Afusion about53.5kDaswe wasfound.The resultsshowthatthe protein expected vitro ofS via recombinantvectorintheE.coli expressedprotein gene plasmid maintains ofTGEV. antigenicity The TGEVS whichwas recombinant andthe TGEV

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