硒对高温胁迫下萝卜幼苗叶绿素含量和酶活性的影响-河南农业科学.pdf

DOI :10.15933/ki.1004 -3268.2010.11.028 2010 年第11期 1 1 2 李亚敏 ,刘建中, 肖红利 (1. , 710069;2. , 712100) :为了研究高温胁迫下硒对萝卜幼苗活性氧代谢保护酶系统的影响,采用盆 试验,将萝卜品种 优白1 号和白玉春 培于不同含硒量的基质中,待萝卜幼苗长至5 ～6cm 时, 移入人工气候室进行高 温胁迫处理(38℃,12h)。结果显示,施硒提高了叶片总叶绿素含量。当施硒量为0.6mg/kg 时, 优白 1 号、白玉春叶片的过氧化物酶(POD)活性分别提高了8.4%和4.3%, 谷胱甘肽过氧化物酶 (GPX)活性分别提高了5.8 倍和1.6 倍。超氧化物歧化酶(SOD)活性却降低了18.4%和20.6% (0.9mg/kg)。由此可见, 一定量的硒可以缓解高温胁迫对萝卜叶片的伤害,提高其耐热性。 :硒(Se);萝卜;高温胁迫;叶绿素;酶活性 :S631.1　　:A 　　:1004-3268(2010)11-0094-04 Effect of Se to horophyll ontent and Enzyme Activity of Radish Seedlings under the High Temperature 1 1 2 LI Ya-min , LIU Jian-zhong , XIAO Hong-li (1.The Science and Technology Department, Shaanxi Radio TV University,Xian 710069, hina; 2.ollege of Life Science, Northwest A F University, Yangling 712100, hina) Abstract:The research aimed to study the effects of high temperature damage on the activated ox- ygen metabolism protective enzyme system .Two cultivars (Youbai1 and Baiyuchun )of radish (R ap han us sat iv us L.)were planted in culture media with different selemium (Se)concentra- tions.When the radish seedlings growed to 5-6cm, the plants were transferred into climatic room and exposed to high temperature strss (38℃, 12h ).Then the leaves were harvested for measurement of chlorophyll and malondialdehyde (MDA )contents and activities of three antioxi- dant enzymes.The results indicate:selenium improved the content of total chorophyll, and in- cresed the activities of peroxidase (POD)and glutathione peroxidase up to 8.4%,4.3%and 5.8, 1.6 times respectively when selenium was 0.