碱性纤维素酶发酵条件的研究-林产化学与工业.pdf

24 3 Vol. 24 No. 3 2004 Chemistry and Industry of Forest Products Sept. 2004 舒 勤, 夏黎明* ( 浙江大学 化学工程与生物工程系, 浙江杭州310027) SHU Q : Bacillus sp. AC- 2 , 24 h; 37 , pH 10. 5; 1% 2% , 76. 04 U/ mL 60 ; 70 pH 6. 0~ 10. 0 : ; ; ; :T Q 25; Q556 :A :0253- 24 17(2004) 03- 0057- 04 ST U DY ON FERMENT AT ION CONDIT IONS FOR PRODUCING AL KALINE CELLULASE BY BA CIL L US SP . AC-2 ST RAIN SHU Qin, XIA L-i ming (Dep artment of Chemical Eng ineering and Bioeng ineer ing, Zhej iang Uni ersity , H angz hou 310027, China) Abstract: Fermentation conditions for producing alkaline cellulase by Bacillus sp. AC-2 were studied in shaking flasks strain. Results showed that the suitable inoculum age w as 24 h. Optimal culture temperature and initial pH val- ue w ere 37 and 10. 5, respectively .The maximal alkaline cellulase activity reached 76.04 U/ mL under the opti- mal conditions with 1% starch and 2% bean cake as carbon and nitrogen source, respectively.T he enzyme prepara- tions w ere stable betw een pH value 6. 0 to 10.0 and below 70 .The reaction catalyzed by alkaline cellulase at pH value . 5 showed an optimum temperature of 60 . This enzyme would have a good potential application in text ile biofinishing and pulp-deinking . Key words: alkaline cellulase; fermentation; textile biofinishing; pulp-deinking , , pH , , [ 1] , , [2] , [3~ 4] Bacillus sp. AC- 2 , , : 2003- 07- 21 : ( 1 7 - ) , , , , * 58