TheeffectofImmunomodulatorybySpirulina螺旋藻对于-中兴大学.docVIP

Title：The effect of Immunomodulatory by Spirulina(螺旋藻對於免疫調節功能的影響) Speaker:賴柏宏 Moderator：顏旭敏 Data Time：2013.06.16(Sun) 08:50～09:12 The order of reporting：1 1.Abstract This research used Spirulina as samples to feed BALB/c mice. BALB/c mice were separated into two groups which were the non-specific immune response group and the specific immune response group. And each group consisted of five minor groups, which were three groups with different doses, comparison group and control group. After four weeks feeds, the non-specific immune response group was used to analyze mices immune about cytokines, antibodies, macrophages, natural killer cells, spleen cells cultures and cells proliferation responses. After the specific immune response group was sensitized by OVA, observing its specific antibodies, cytokines and cells proliferation responses. The result showed that the mice which are fed with Spirulina could significantly increase the quantities of specific antibodies IgM, IgG, also the activity of macrophages and natural killer cells. All in all, Spirulina can help mices immune to increase. 2. Introduction 藍藻(blue-green algae)中有一種形狀為螺旋狀者，而被稱為螺旋藻(Spirulina)外觀為螺旋狀並因含有藻藍素(phycocyanin)、葉綠素(chlorophyll)，而呈現藍綠色。螺旋藻的營養價值極高，含有的蛋白質達60-70%，且人體無法合成的必需胺基酸所含的比例高；富含維生素B12、β-胡蘿蔔素，γ-亞麻油酸的含量亦高。螺旋藻含有豐富的礦物質及錳、鋅等微量元素(翁婉琳，2008)。Ayehunie et al. 的研究指出螺旋藻的水溶性萃取物(Aqueous extract of Spirulina platensis)可以抑制人類免疫缺乏病毒(Human immunodeficiency virus type Ⅰ, HIV-1)在T細胞、週邊血液單核性細胞(Peripheral blood mononuclear cells,PBMC)、蘭格罕細胞(Langerhans cells)中的複製，對於免疫反應有相當大的幫助。 實驗設計主要針對非特異性免疫及特異性免疫反應動物模式來進行研究，期中包括利用OVA來誘發特異性免疫反應，進一步測定OVA特異性的抗體濃度、OVA特異性的增殖反應和細胞激素製造的情形等。同時，為了解螺旋藻是否會影響到非特異性的免疫反應，如：自然殺手細胞活性、吞噬細胞活性，亦同時進一步加以分析。本試驗藉由非特異性免疫反應與特異性免疫反應的相關指標，評估螺旋藻的影響，可得較完整而具體的免疫調節的功能評估。 3.Materials and methods 測定OVA專一性抗體的ELISA、脾臟細胞增生反應、吞噬細胞活性測定、自然殺手細胞活性測定 4.Results and discussion 4.1測定OVA專一性抗體的ELISA結果 血清中OVA特異性抗體效價部分，隨著餵食劑量增加，OVA致敏小鼠血清中的OVA特異性IgM及IgG抗體在0.5X、1X、2X劑量中有增加的趨勢，其中IgM增加反應趨勢，1X之劑量有顯著差異；IgG增加反應趨勢，0.5X、1X、2X之劑量皆有顯