中国肿瘤临床基因芯片技术及其在骨肉瘤耐药机制研究中的应用.PDF

1608 中国肿瘤临床 2011 年第38 卷第24 期 基因芯片技术及其在骨肉瘤耐药机制研究中的应用 周宏明 综述 王玉学 审校 摘要 基因芯片技术打破了以往陈旧的研究模式，从系统宏观地研究生物整体基因的表达及功能。骨肉瘤患者化疗过程 中肿瘤细胞对化疗药物产生耐药性是引起化疗失败的重要原因之一，其机制复杂，由肿瘤的综合特性决定，对药物的摄取减少和 外排增多、DNA 损伤修复能力的增强、抗调亡潜能的提高、细胞膜通透性的降低和相关酶活性的变化等都会导致骨肉瘤细胞对化 疗药物产生耐药性。利用基因芯片技术从基因水平对骨肉瘤耐药机制进行研究，可以快速检测大量耐药相关基因，作为深入研 究骨肉瘤化疗耐药机制有效手段。本文分别对基因芯片的原理、分类和特点，骨肉瘤耐药机制和基因芯片技术在其中的应用进 行综述。 关键词 骨肉瘤 化疗 耐药 基因芯片 doi ：10.3969/j.issn. 1000-8179.2011.24.034 Gene Chip Technology and Its Use in the Research on Drug Resistance Mechanism of Osteosarcoma Hongming ZHOU, Yuxue WANG Correspondence to: Yuxue WANG, E-mail: wyx_hyd@ Department of Orthopedic Surgery, Cancer Hospital of Harbin Medical University, Harbin 150081, China Abstract Microarray (gene chip) technology has broken the old research model and has been used to study the system of gradu- ate macro expression and function of the object. This process is possible through integrated molecular biology, laser chemistry, comput- er science, and other disciplines related to technology. Gene chip has a high throughput, high sensitivity, high specificity, and other par- allel characteristics. Osteosarcoma is one of the most common malignant bone tumors. In the last 20 to 30 years, the treatment of osteo- sarcoma has made significant progress. A number of studies have shown that integrated chemotherapy can improve the survival rate of patients with osteosarcoma from 20%-30% to 60-70%. Therefore, palliative chemotherapy is no longer a passive treatment, but an im- portant way to reduce tumor recurrence rate, improve the quality of life, and prolong survival. However, the resistance of osteosarcoma cells to chemotherapeutic drug is one of important reasons for chemotherapy failure. Drug resistance is a complicated mechanism, which is decided by the integrated features of tumors, such as the re