毕业论文--不同姜汁对大鼠乙醇致胃黏膜损伤的研究.docxVIP

毕业论文 PAGE 1 目? 录 1、标题和作者………………………………………………1 2、中文摘要及关键词………………………………………3 3、英文摘要及关键词………………………………………3 4、前言………………………………………………………4 5、正文………………………………………………………5 6、讨论或小结………………………………………………8 7、参考文献…………………………………………………8 8、文献综述…………………………………………………9 9、致谢及声明………………………………………………14 不同姜汁对大鼠乙醇致胃黏膜损伤的研究 中文摘要 目的：探讨姜汁对急性胃黏膜损伤的保护作用及其炎症反应的抑制机制，及其不同姜汁之间的差异性。方法：空白组、模型组、生姜榨汁组、生姜煮汁组和干姜煮汁组，采用无水乙醇灌胃制急性胃黏膜损伤模型，按文献打分法计算胃黏膜损伤指数（UI），HE染色观察胃黏膜组织形态学变化，放免法检测血清中TNFα、IL-8、6-keto-PGF1α的含量。结果：与模型组比较，生姜煮汁、生姜榨汁、干姜煮汁的损伤指数明显减少（P0.01），胃黏膜组织形态较为完好；与生姜煮汁比较，生姜榨汁、干姜煮汁在损伤指数减少上存在显著性差异（P0.05）。与模型组比较；生姜榨汁、生姜煮汁和干姜煮汁组中6-keto-PGF1α的含量在数值降低上有显著性差异。（P0.01;P0.05;P0.05）。结论：生姜榨汁、生姜煮汁及干姜煮汁均能减轻大鼠的胃黏膜破损，抑制炎症的浸润，生姜榨汁效果最好，这可能与6-keto-PGF1α的含量有关。 关键词：姜汁；胃黏膜损伤；大鼠 ABSTRACT Purpose：To investigate the protective effect of ginger juice on acute gastric mucosal injury and the mechanism of the inhibition of the inflammatory response, and the difference between different ginger juice. Methods：Blank group, model group, ginger juice group, ginger, cook juice group and dried ginger boiled juice group, using ethanol lavage stomach model of acute gastric mucosal lesion, gastric mucosal ulcer index (UI) is calculated according to the literature method of scoring, he staining was used to observe the morphological changes of gastric mucosa, put immunoassay of serum TNF, IL-8, 6-Keto-PGF1a content.Result：Compared with the model group, the ginger boiled juice, ginger juice, ginger boiled juice of damage index was significantly decreased (P 0.01), morphology of gastric mucosal tissue is intact; and ginger boiled juice comparison, ginger juice, ginger boiled juice damage index reduce exist significant difference (P 0.05) in. Compared with the model group; ginger juice, ginger, cook juice and dried ginger boiled juice group 6-Keto-PGF1a content; there are significant difference in reducing the numerical. (P0.01; P0.05; P0.05).Conclusion：Ginger juice, ginger boiled and dried ginger juice boiled juice can reduce the rat gastric mucosa damage, inhibit inflam