甘氨酰trn诗a合成酶调节奶牛乳腺上皮细胞nfκb1信号通路研究.docxVIP

目录4．3 目录 4．3 p-GlyRS与GCN2／elF2a途径的关系 ．45 4．4蛋白质翻译和mTOR途径对GIyRS磷酸化的影响 45 5结论 ．47 致谢 ．．48 参考文献 49 附录 ．．57 攻读硕士学位期间发表的学术论文 。58 111 万方数据 C0brrENTSCoNTENTS C0brrENTS CoNTENTS Abstract in Chinese ．．． ．I Abstract in English ．．．．．． ．． ．．． ． ．．．．． ． ． ．III 1 Introduction 1 1．1 Development and lactation ofbovine mammary gland ．1 1．2 Research of bovine milk protein synthesis mechanism 1 1．3 Research of signal transduction pathway of bovine milk protein synthesis 2 1．3．1 JAK2-STAT5 signaling pathway ．．．．．． ．．．．． ．．．．．．．．．． ”2 1．3．2 mTOR signaling pathway ．．．．．． ．．． ．．． ．．． ．．．．． ．．．． ．．．．．．． 3 1．4 Regulation of lactation by amino acids 4 1．5 The relative research ofNF．，cB ．．4 1．5．1 The classification ofNF．r,B ．4 1．5．2 The function research ofNF．1cB ．5 1．6 Research offunction ofaminoacyl tRNA synthase ． ．． ．．．．．．．．．6 1．6．1 The structure and function of aminoacyl tRNA synthase 6 1．6．2 The relative research ofGlycine tRNA Synthetase ．．7 1．7 The relative research ofGCN2／eIF2a pathway 7 1．7．1 The relative research ofelF2a 7 1．7．2 The relative research of GCN2 ． ． ．．．． ． ．．．． ．。8 1．8 Purpose and significance ofthe research ． ．．．．．．． ． ． ．．．． ．．．．．．．．．．．．．． ．．．． ．8 2 Materials and methods． ． ． ． ．．． ．． ．．．．．．．．．．．． ．． ．．．9 2．1 Materials．．．．．．．．．． ．．．． ． ．．．．． ．．．．．． ．．．．．． ．．．．．．．．．．．．．．．．．．．． ．．9 2．J．J Reagents 9 2．1．2 Instruments ． ． ．．．．．．．10 2．1．3 Animals and cells ． ． ．．．．．．．．．．．．．． ．． ．．． ．．．． ．10 2．1．4 Reagent preparation ．．10 2．2 Cultivation purification and identification ofBMECs 14 2．2．1 Primary cultivation and purification ofBMECs ．．14 2．2．2 Identification ofBMECs ． ．．．．．．． ．．． ．