硕士研究生毕业论文三七药材及提取物农药残留测定分析.docVIP

XXXX医药大学XXXX年硕士研究生毕业论文 PAGE PAGE 1 硕士研究生毕业论文 三七药材及提取物 农药残留测定分析 XXXX年XX月XX日 中文摘要 三七是我国的名贵中药，为了有效地监测其农药残留情况，控制其内在质量，满足国际市场的要求，本论文建立了三七药材及其提取物和制剂的农药残留分析方法，并制定相应的限度标准，同时为其他中药建立通用的农药残留分析方法与严格完善的农药残留限量标准提供科学依据与方法借鉴，提高中药品质，增强中药在国际市场上的竞争力，使中药早日全面进入国际市场。 本论文结合国内外文献，运用气相色谱-质谱联用技术，建立了三七药材、三七药材提取物三七三醇皂苷及三七通舒胶囊中四大类20种农药同时测定的定性定量分析方法。首先对色谱柱、进样口温度、特征离子、离子源温度等进行优选研究，确定了色谱、质谱条件，然后再通过对样品提取溶剂、提取方法和净化方法的实验研究，确定了三七药材、三七三醇皂苷原料药及三七通舒胶囊的样品前处理方法，之后对建立的方法进行方法学验证，最后运用建立的分析方法分别对15个批次的三七药材、15个批次的三七三醇皂苷原料药及15个批次的三七通舒胶囊进行20种农药残留量的检测。 结果表明，实验所建立的20种农药残留测定的分析方法准确灵敏、稳定可靠，重复性、专属性和耐用性均很好，可控性强。15批PTS和15批三七通舒胶囊均未检出20种农药，而三七药材中农药残留现象较为普遍，15批药材有不同程度的农药残留检出。 关键词：三七药材；三七三醇皂苷；三七通舒胶囊；农药残留；气相色谱-质谱联用；固相萃取。 ABSTRACT Panax Notoginseng is a very valuable medicine in China.To monitor its pesticide residues effectively,control its inner quality,meet the requirements of international markets,this thesis established methods of analysis for pesticide residues in Panax Notoginseng,its extract and its herbal preparation,and made limit standards.At the same time,it provided scientific basis and mothods reference for the establishment of universal methods of analysis for pesticide residues and strict,complete limit standards in other Chinese materia medica,improved the quality of Chinese materia medica,enhanced the competitiveness of Chinese materia medica in the international markets and made Chinese materia medica fully enter international markets as soon as possile. This thesis incorporated the literatures at home and abroad, made use of gas chromatography-mass spectrometry(GC-MS),to develop methods of qualitative and quantitative analysis simultaneously for 20 pesticide residues in Panax Notoginseng, its extract PTS and SanQiTongShu Capsules.First,we studied and optimized the chromatographic columns,injection temperature,characteristic ions,ion source temper- ature during the experiment,built the GC and MS condition.Then we studied the extra- ction solvents, extraction methods and purification methods of sample a