生物保鲜剂对鱼类腐败菌抑菌效果比较及抑菌机理研究.pdf

※基础研究 食品科学 2012, Vol.33, No.23 31 1,2 2 2, * 2 蒋慧亮 ，李学英 ，杨宪时 ，迟 海 (1.江苏畜牧兽医职业技术学院食品科技学院，江苏 泰州 225300 ；2. 中国水产科学研究院东海水产研究所，上海 200090) 摘 要：通过分析抑菌圈直径和最低抑制量，研究葡萄柚种子提取物、葡萄籽提取物、柠檬提取物、百里酚和茶多 酚5种生物保鲜剂对假单胞菌和腐败希瓦氏菌的抑菌效果进行比较。结果表明：百里酚、茶多酚和葡萄籽提取物表 现出较好的抑菌效果，其中百里酚对两种菌都有很好的抑菌效果，尤其是对腐败希瓦氏菌抑菌效果显著。茶多酚对 两种菌的抑菌效果相当，而葡萄籽提取物则对假单胞菌的抑菌效果更好，总体上百里酚的抑菌效果较好。通过测定 菌液电导率、可溶性糖含量和蛋白质含量，分析百里酚对假单胞菌和腐败希瓦氏菌的抑菌机理。结果表明：百里酚 处理组的菌液电导率、可溶性糖含量和蛋白质含量均比对照组大，百里酚的抑菌机理可能是：增大细胞膜通透性， 破坏细胞膜外膜完整性，破坏磷脂双层。 生物保鲜剂；假单胞菌；腐败希瓦氏菌；抑菌效果；百里酚；抑菌机理 Comparative Antibacterial Effects and Mechanisms of Biological Preservatives against Fish Spoilage Organisms 1,2 2 2, * 2 JIANG Hui-liang ，LI Xue-ying ，YANG Xian-shi ，CHI Hai (1. School of Food Science and Techonoly, Jiangsu Animal Husbandry and Veterinary College, Taizhou 225300, China； 2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China) Abstract ：The antibacterial effects of fi ve biological preservatives including grapefruit seed extract, grape seed extract, lemon extract, thymol and tea polyphenols against Pseudomonas spp. and Shewanella putrefacien as specific spoilage organisms (SSOs) in fi sh were comparatively investigated by measuring inhibitory circle diameter and minimum inhibitory concentration (MIC). The results showed that thymol, tea polyphenols and grape seed extract had strong antibacterial effect. Meanwhile, thymol revealed extremely strong antibacterial effects against both spoilage organisms, especially Shewanella putrefacien. Tea polyphenols