图像细胞检测法在酿酒酵母快速计数和生理活性分析中的应用.pptx

图像细胞检测法在酿酒酵母快速计数和生理活性分析中的应用Novel image cytometric method for detection of physiological and metabolic changes in Saccharomyces cerevisiaeRuwei LiPh.DFAS, BDNexcelom Bioscience10 years – design, manufacture and market cell enumeration and analysis systems with image cytometry technologiesOur approach: scientists help scientists to reduce error and increase assay accuracy50% employees with science degree, 8 field application scientists in US, 4 FAS in China and still growingWork with lead labs in each research field to develop productsSpread best practice to other labsNexcelom Bioscience 的创新之路Nexcelom 全球酵母生理特性在发酵过程中显得至关重要Essential for brewing, wine, and baking industriesViability, vitality, glycogen, neutral lipid, and trehalose contentOptimize fermentation processCan improves qualityCan improves consistency传统的酵母生理活性检测方法Manual counting methodTraditional method Methylene blue for “viability”Prone to user-variationTime-consumingFluorescence MicroscopyAbility to study fluorescenceImage-based observationLacks automationQualitative analysisFlow CytometryProvides automationImproves statistical analysisConsiderable maintenanceProne to cloggingCross contaminationLack of imaging capabilityCellometer 图像细胞仪在科研和工业酵母中的应用Yeast concentration and viability measurementJIMB 38:8, 1109-1115JIMB 39:11, 1615-1623Strain developmentFEMS Microbio. Eco. 80:3, 578-590Int. J Food Microbio. 157:1, 45-51Monitoring production qualityBioControl 57, 451-461FEMS Microbio. Eco. 76:1, 145-155Cellometer 图像细胞仪工作原理操作步骤2. Pipette 20uL into counting chamber 3. Insert chamber into instrument4. Select assay and click count1. Stain sample5. Bright field and fluorescent images are acquired and analyzed6. Cell count / viability are calculated automatically专业的模式识别软件对酵母形态的精确识别单细胞状态Enumerate single-celled particlesDisregard debris or clumps of cells by sizing parameters出芽酵母Image analysis algorithm decluster and enumerate single yeast cells链状酵母Enumerate chain-forming single yeast cells在啤酒和白酒酿造中的应用Sample nameConcentr