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2.2 病毒核酸提取和cDNA 制备······························································31
2.3 盖塔病毒基因序列扩增和测定····························································31
2.4 盖塔病毒序列整理和数据集构建31
3 实验结果32
3.1 GETVYN12042 病毒株的全基因组序列················································33
3.2 GETV 分析数据集的时间和地域分布···················································33
3.3 GETV 分析数据集的宿主媒介分布······················································33
4 讨论34
5 小结35
第二节 盖塔病毒的种群特征和时空动力学分析················································35
1 实验材料36
1.1 DNAStar 软件,ClustalX 2.0.9,Bioedit,Mega 6.06软件 ·····························36
1.2 GeneDoc 软件·················································································36
1.3 BEASTv1.7.2 软件···········································································36
1.4 Tracerv1.5 软件37
1.5 FigTreev1.3.1软件···········································································37
1.6 GoogleEarth v6.02············································································37
1.7 SWISS-MODELonlineserver······························································37
1.8 VMD software··················································································37
1.9 YASARAsoftwares37
2 实验方法38
2.1 盖塔病毒数据集的序列分析·······························································38
2.2 系统进化及种群动态分析··································································38
2.3 空间动力学分析 ·············································································38
2.4 氨基酸结构分析38
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