拟除虫菊酯类农药多残留的IC-ELISA检测方法研究-食品安全检测.PDF

食品科技 2008 年第33 卷第 12 期 FOOD SCIENCE AND TECHNOLOGY 食品安全与检测 拟除虫菊酯类农药多残留的 IC-ELISA 检测方法研究 杨 挺， 皇甫伟国， 谢显传 (农业部农产品质量安全监督检验测试中心(宁波) ，宁波315040) 摘要： 为检测样品中拟除虫菊酯类农药多残留，以间苯氧基苯甲酸(PBA)为半抗原，用活性酯法 将PBA 与牛白蛋白(BSA)偶联，制备多克隆抗体，并建立间接竞争酶联免疫吸附测定法。用方阵 滴定确定包被抗原的最适工作浓度为5.0 μg/mL ，抗体最佳浓度为10.0 μg/mL ，该方法的最低检 测限为0.301 μg/mL ，在0.1～200 μg/mL 工作浓度范围内，标准曲线表现良好的线性关系。 关键词： ELISA ；拟除虫菊酯；农药；残留分析 中图分类号 + 文献标志码： 文章编号 ：TS 207.5 3 A ：1005-9989(2008)12-0291-04 Indirect competitive ELISA (IC-ELISA) for multiresidues detection of the pyrethroids YANG Ting, HUANGFU Wei-guo, XIE Xian-chuan (Supervision, Inspection Testing Center of Agricultural Products Quality Security, Ministry of Agriculture(Ningbo), Ningbo 315040) Abstract : In order to detect multiresidues of pyrethroids, 3-phenoxybenzoic acid(PBA), which was used as protein carrier to BSA, was used as genetic hapten and in order to generate broad specificity antibody against a group of pyrethriod insecticides. Indirect competitive ELISA(IC-ELISA) was established ． The most appropriate titration of coating antigen was 5.0 μg/mL and optimal antibody was 10.0 μg/mL correspondingly． The standard curve of couple with semi -antigen by active ester method ． IC -ELISA was also established and the curve indicated that the lowest detection limit was 0.301 μg/mL，the curve had a favorable linear relation with the concentration range of 0.1～200 μg/mL. Key words: ELISA; pyrethroids; pesticide; residue analysis [1-4] 拟除虫菊酯类农药对刺