番茄果实中全反式番茄红素的C30-HPLC外标法定量检测.pdf

※分析检测 食品科学 2010, Vol. 31, No. 22 453 丁 靖，惠伯棣 * ，刘 源 (北京联合大学应用文理学院，北京 100191) 为建立一种在 C30-HPLC 上使用外标法对番茄红素全反式异构体进行定量检测的方法，采用色谱条件：固 定相：YMC Carotenoid columu C30 色谱柱(4.6mm ×250mm ，SOD-5 μm ，Waters) ；流动相A ：乙腈- 甲醇(3:1 ， V/V) ；流动相B ：甲基叔丁基醚(MTBE)；流动相A 与 B 中分别添加0.05%( V/V) 的三乙胺；线性梯度洗脱：B 在 20min 内由0 增至 80%，20～35min 内B 维持在 80%；流速：1mL/min；色谱图检测波长470nm ；进样量：20 μL ； 柱温：室温。结果表明：此方法的最小定量限为 0.10 μg/mL ，在 10～100 μg/mL 范围内质量浓度与组分峰面积的 2 线性回归方程为y =212181x ，R =0.9738 。相对标准偏差为4.04% 。外标加样回收率为97.34%(RSD 为 0.904%) 。应 用本方法可对番茄果实中的全反式番茄红素进行定量分析。 全反式番茄红素；定量分析；外标；C 30 -HPLC Quantification of All-trans-lycopene from Tomato Fruits by C30-HPLC with External Standard Method DING Jing ，HUI Bo-di* ，LIU Yuan (College of Applied Arts and Science, Beijing Union University, Beijing 100191, China) Abstract ：An external standard method was established to determine all-trans-lycopene on C30-HPLC. Stationary phase was YMCTM Carotenoid column C30 (4.6 mm × 250 mm, SOD-5 μm, Waters); mobile phase A was acetonitrile-methanol (3:1, V/ V); mobile phase B was methyl tert-butyl ether (MTBE); both mobile phase A and B were added 0.05% ( V/V) triethylamine; linear gradient elution was conducted with an increase of mobile phase B from 0 to 80% ( V/V) in 20 min followed by maintaining at 80% mobile phase B for 15 min; flow rate was 1.0 mL/min; monitoring wavelength was 470 nm; injection volume was 20 μL; column temperature was set at room temperature. Under these determination parameters, the minimum detection limit was 0.10 μg/mL; in the range of 10 －100 μg/mL, linear correlation equation was y = 212181x (R2 = 0.9738) with a RSD of 4.